Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27511 | 3' | -57.3 | NC_005879.1 | + | 33891 | 0.66 | 0.521104 |
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Target: 5'- cGAGGAGUcaauGCuCGCCGccCAUCUACGc- -3' miRNA: 3'- cCUCCUCG----CG-GCGGCa-GUAGGUGUug -5' |
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| 27511 | 3' | -57.3 | NC_005879.1 | + | 19587 | 0.66 | 0.52004 |
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Target: 5'- gGGAGccGCGCCGCCauuaaauGUCAauguUCCGCcugGACu -3' miRNA: 3'- -CCUCcuCGCGGCGG-------CAGU----AGGUG---UUG- -5' |
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| 27511 | 3' | -57.3 | NC_005879.1 | + | 19890 | 0.66 | 0.500002 |
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Target: 5'- aGGAGGAagaccauuuGUGCCGUCcUCG-CCGCGAa -3' miRNA: 3'- -CCUCCU---------CGCGGCGGcAGUaGGUGUUg -5' |
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| 27511 | 3' | -57.3 | NC_005879.1 | + | 12417 | 0.66 | 0.498957 |
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Target: 5'- aGGAaucccuuGGAcUGCCGCCGUCc-CCugAACa -3' miRNA: 3'- -CCU-------CCUcGCGGCGGCAGuaGGugUUG- -5' |
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| 27511 | 3' | -57.3 | NC_005879.1 | + | 20699 | 0.69 | 0.356485 |
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Target: 5'- uGGAuuGGAGCGCCGUCGacCAaCCACu-- -3' miRNA: 3'- -CCU--CCUCGCGGCGGCa-GUaGGUGuug -5' |
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| 27511 | 3' | -57.3 | NC_005879.1 | + | 10629 | 1.11 | 0.000315 |
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Target: 5'- cGGAGGAGCGCCGCCGUCAUCCACAACc -3' miRNA: 3'- -CCUCCUCGCGGCGGCAGUAGGUGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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