Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27516 | 5' | -52.6 | NC_005879.1 | + | 21930 | 0.66 | 0.796723 |
Target: 5'- --cUGACGUauACACUCGCGcuGCGCGCg -3' miRNA: 3'- acaGCUGCAgcUGUGGGUGU--UGUGUG- -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 1148 | 0.66 | 0.765781 |
Target: 5'- gUGUCGACGUCGACACagguuuCGAUu--- -3' miRNA: 3'- -ACAGCUGCAGCUGUGggu---GUUGugug -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 38042 | 0.7 | 0.552777 |
Target: 5'- gUGUCGACgGUCGACACaCCcCGA-ACAUg -3' miRNA: 3'- -ACAGCUG-CAGCUGUG-GGuGUUgUGUG- -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 9012 | 0.72 | 0.445633 |
Target: 5'- gUGUCGAUGUCGACAuacuUCCGCu-UugGCg -3' miRNA: 3'- -ACAGCUGCAGCUGU----GGGUGuuGugUG- -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 7048 | 0.72 | 0.42559 |
Target: 5'- gUGUCGAUGUCGACACaCCcUAAU-CACc -3' miRNA: 3'- -ACAGCUGCAGCUGUG-GGuGUUGuGUG- -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 36222 | 0.73 | 0.368833 |
Target: 5'- gUGUCGACGUCGACACCgC-CuuUGCGa -3' miRNA: 3'- -ACAGCUGCAGCUGUGG-GuGuuGUGUg -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 33285 | 0.74 | 0.342466 |
Target: 5'- gUGUCGACGUCGACACaCUgguaGCGAaAUACc -3' miRNA: 3'- -ACAGCUGCAGCUGUG-GG----UGUUgUGUG- -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 24632 | 0.76 | 0.271708 |
Target: 5'- gUGUCGACGUCGACACCCu-------- -3' miRNA: 3'- -ACAGCUGCAGCUGUGGGuguugugug -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 29333 | 0.76 | 0.271708 |
Target: 5'- gUGUCGACGUCGACACCCu-------- -3' miRNA: 3'- -ACAGCUGCAGCUGUGGGuguugugug -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 36276 | 0.76 | 0.237739 |
Target: 5'- gUGUCGACGUCGACACaCUGCcgaGCAUc -3' miRNA: 3'- -ACAGCUGCAGCUGUG-GGUGuugUGUG- -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 1094 | 0.77 | 0.207397 |
Target: 5'- gUGUCGACGUCGACACUauuccCGAUGCAg -3' miRNA: 3'- -ACAGCUGCAGCUGUGGgu---GUUGUGUg -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 34815 | 0.79 | 0.170583 |
Target: 5'- gUGUCGACGUCGACACCCuACuuaaggagcCAgACa -3' miRNA: 3'- -ACAGCUGCAGCUGUGGG-UGuu-------GUgUG- -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 29387 | 0.8 | 0.147975 |
Target: 5'- gUGUCGACGUCGACACCCuCuuuuuACugGa -3' miRNA: 3'- -ACAGCUGCAGCUGUGGGuGu----UGugUg -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 34761 | 0.8 | 0.131891 |
Target: 5'- gUGUCGACGUCGACACCCAUc------ -3' miRNA: 3'- -ACAGCUGCAGCUGUGGGUGuugugug -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 16894 | 0.81 | 0.120902 |
Target: 5'- gUGUCGACGUCGACACCCucccccuCAgGCu -3' miRNA: 3'- -ACAGCUGCAGCUGUGGGuguu---GUgUG- -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 21856 | 0.82 | 0.104462 |
Target: 5'- gUGUCGACGUCGACACCCuaucuCAccGCucuCACu -3' miRNA: 3'- -ACAGCUGCAGCUGUGGGu----GU--UGu--GUG- -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 16840 | 0.82 | 0.101437 |
Target: 5'- gUGUCGACGUCGACACCCcCGA-ACAg -3' miRNA: 3'- -ACAGCUGCAGCUGUGGGuGUUgUGUg -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 21802 | 0.84 | 0.0777 |
Target: 5'- gUGUCGACGUCGACACCCGCccauuAUuuGCa -3' miRNA: 3'- -ACAGCUGCAGCUGUGGGUGu----UGugUG- -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 33231 | 0.9 | 0.030796 |
Target: 5'- gUGUCGACGUCGACACCCaucauuucagcagcuACAGCAUAUa -3' miRNA: 3'- -ACAGCUGCAGCUGUGGG---------------UGUUGUGUG- -5' |
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27516 | 5' | -52.6 | NC_005879.1 | + | 24686 | 1.11 | 0.000984 |
Target: 5'- gUGUCGACGUCGACACCCACAACACACa -3' miRNA: 3'- -ACAGCUGCAGCUGUGGGUGUUGUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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