Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27520 | 3' | -52.9 | NC_005879.1 | + | 22603 | 0.66 | 0.786245 |
Target: 5'- cGGGgGUuccUGACGUgGACGCCUU-CUUGg -3' miRNA: 3'- -CCCaCA---GCUGCAgCUGUGGGAuGAAU- -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 29657 | 0.66 | 0.775966 |
Target: 5'- uGGGUGUUGAgGcguuUCGACACUUUgauuugcgACUUAg -3' miRNA: 3'- -CCCACAGCUgC----AGCUGUGGGA--------UGAAU- -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 9013 | 0.68 | 0.677819 |
Target: 5'- -uGUGUCGAUGUCGACAUaCUuccGCUUu -3' miRNA: 3'- ccCACAGCUGCAGCUGUGgGA---UGAAu -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 34214 | 0.68 | 0.643822 |
Target: 5'- uGGGUGUUGuAUGUCGAaaCACCCcaaggGCa-- -3' miRNA: 3'- -CCCACAGC-UGCAGCU--GUGGGa----UGaau -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 14591 | 0.69 | 0.598382 |
Target: 5'- uGGUGUUGAgGUCGACcguaccauugacGCCCUAg--- -3' miRNA: 3'- cCCACAGCUgCAGCUG------------UGGGAUgaau -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 1149 | 0.69 | 0.575805 |
Target: 5'- -aGUGUCGACGUCGACACa------- -3' miRNA: 3'- ccCACAGCUGCAGCUGUGggaugaau -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 38044 | 0.72 | 0.426676 |
Target: 5'- uGGUGUCGACgGUCGACACaCCc----- -3' miRNA: 3'- cCCACAGCUG-CAGCUGUG-GGaugaau -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 1093 | 0.73 | 0.379131 |
Target: 5'- -uGUGUCGACGUCGACACUauucccgaUGCa-- -3' miRNA: 3'- ccCACAGCUGCAGCUGUGGg-------AUGaau -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 37983 | 0.74 | 0.318795 |
Target: 5'- gGGuGUGUCGAcCGUCGACACCaUugUUc -3' miRNA: 3'- -CC-CACAGCU-GCAGCUGUGGgAugAAu -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 36221 | 0.76 | 0.232729 |
Target: 5'- -uGUGUCGACGUCGACACCgccuUUGCg-- -3' miRNA: 3'- ccCACAGCUGCAGCUGUGG----GAUGaau -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 6990 | 0.76 | 0.232729 |
Target: 5'- gGGuGUGUCGACaUCGACACaCCUAUUUu -3' miRNA: 3'- -CC-CACAGCUGcAGCUGUG-GGAUGAAu -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 7052 | 0.78 | 0.186877 |
Target: 5'- aGGuGUGUCGAUGUCGACacACCCUAa--- -3' miRNA: 3'- -CC-CACAGCUGCAGCUG--UGGGAUgaau -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 33230 | 0.82 | 0.105491 |
Target: 5'- -uGUGUCGACGUCGACACCCa----- -3' miRNA: 3'- ccCACAGCUGCAGCUGUGGGaugaau -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 36278 | 0.84 | 0.071875 |
Target: 5'- cGGUGUCGACGUCGACACaCUGCc-- -3' miRNA: 3'- cCCACAGCUGCAGCUGUGgGAUGaau -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 33288 | 0.84 | 0.069765 |
Target: 5'- uGGGUGUCGACGUCGACACaCUGgUa- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGgGAUgAau -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 16837 | 0.92 | 0.019548 |
Target: 5'- aGGGUGUCGACGUCGACACCCccgaacaggucgUGCUa- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGG------------AUGAau -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 34758 | 0.93 | 0.017294 |
Target: 5'- aGGGUGUCGACGUCGACACCCa----- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGaugaau -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 21799 | 0.93 | 0.017294 |
Target: 5'- aGGGUGUCGACGUCGACACCCg----- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGaugaau -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 24689 | 0.94 | 0.014836 |
Target: 5'- aGGGUGUCGACGUCGACACCC-ACa-- -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGaUGaau -5' |
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27520 | 3' | -52.9 | NC_005879.1 | + | 16897 | 0.95 | 0.012725 |
Target: 5'- gGGGUGUCGACGUCGACACCCUcccccucagGCUUc -3' miRNA: 3'- -CCCACAGCUGCAGCUGUGGGA---------UGAAu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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