Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27520 | 5' | -50.7 | NC_005879.1 | + | 11309 | 0.66 | 0.850517 |
Target: 5'- aGGCGgcGGCGgcGGCGguaauagCGCCGUUUGGu -3' miRNA: 3'- -CCGU--UCGUuuCUGUaa-----GCGGCGGAUC- -5' |
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27520 | 5' | -50.7 | NC_005879.1 | + | 1410 | 0.67 | 0.822271 |
Target: 5'- gGGCAGGUAAGGAUAUUggggCGuuGCaCUu- -3' miRNA: 3'- -CCGUUCGUUUCUGUAA----GCggCG-GAuc -5' |
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27520 | 5' | -50.7 | NC_005879.1 | + | 27575 | 0.67 | 0.812388 |
Target: 5'- cGCAAucGCAGGGuuGUaCGCCGCCa-- -3' miRNA: 3'- cCGUU--CGUUUCugUAaGCGGCGGauc -5' |
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27520 | 5' | -50.7 | NC_005879.1 | + | 12657 | 0.68 | 0.737938 |
Target: 5'- aGGCGgaaaGGUGAuuGACA-UCGCCGCCg-- -3' miRNA: 3'- -CCGU----UCGUUu-CUGUaAGCGGCGGauc -5' |
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27520 | 5' | -50.7 | NC_005879.1 | + | 34988 | 0.68 | 0.72671 |
Target: 5'- cGGCAGGUGAgcgcuugauuGGAgAUUCGCUGCUc-- -3' miRNA: 3'- -CCGUUCGUU----------UCUgUAAGCGGCGGauc -5' |
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27520 | 5' | -50.7 | NC_005879.1 | + | 30600 | 0.69 | 0.669244 |
Target: 5'- ----cGuCAAAGGCGUUgGCCGUCUGGg -3' miRNA: 3'- ccguuC-GUUUCUGUAAgCGGCGGAUC- -5' |
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27520 | 5' | -50.7 | NC_005879.1 | + | 17357 | 0.69 | 0.666914 |
Target: 5'- aGGCGAGCGGAGACuaaCGaagucacaacaaCGCCUGu -3' miRNA: 3'- -CCGUUCGUUUCUGuaaGCg-----------GCGGAUc -5' |
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27520 | 5' | -50.7 | NC_005879.1 | + | 12141 | 0.7 | 0.65758 |
Target: 5'- aGGUAAGUugGAAGAUA-UCGC-GCCUAGc -3' miRNA: 3'- -CCGUUCG--UUUCUGUaAGCGgCGGAUC- -5' |
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27520 | 5' | -50.7 | NC_005879.1 | + | 27393 | 0.71 | 0.541397 |
Target: 5'- aGGCGGGUccAGAGGCAaaagccgucaUUGCCGCCUAc -3' miRNA: 3'- -CCGUUCG--UUUCUGUa---------AGCGGCGGAUc -5' |
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27520 | 5' | -50.7 | NC_005879.1 | + | 33834 | 0.74 | 0.383462 |
Target: 5'- cGGCGAGCAuuGACuccUCGCUGCagUAGg -3' miRNA: 3'- -CCGUUCGUuuCUGua-AGCGGCGg-AUC- -5' |
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27520 | 5' | -50.7 | NC_005879.1 | + | 34778 | 1.11 | 0.001383 |
Target: 5'- uGGCAAGCAAAGACAUUCGCCGCCUAGc -3' miRNA: 3'- -CCGUUCGUUUCUGUAAGCGGCGGAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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