miRNA display CGI


Results 1 - 3 of 3 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27522 5' -53 NC_005879.1 + 36320 0.66 0.715288
Target:  5'- gGuuGAGCgaaucggGUcACUCUGUACCGCa -3'
miRNA:   3'- -UggUUCGacaa---CGaUGAGACGUGGCG- -5'
27522 5' -53 NC_005879.1 + 11982 0.66 0.692556
Target:  5'- aAUCAGGCguccUGUguugGCUGCacCUGCAUCGUc -3'
miRNA:   3'- -UGGUUCG----ACAa---CGAUGa-GACGUGGCG- -5'
27522 5' -53 NC_005879.1 + 920 1.13 0.000519
Target:  5'- aACCAAGCUGUUGCUACUCUGCACCGCa -3'
miRNA:   3'- -UGGUUCGACAACGAUGAGACGUGGCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.