Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27526 | 3' | -50.9 | NC_005879.1 | + | 21179 | 0.66 | 0.79347 |
Target: 5'- -gAUGGCACUagccgUGGCU-CCGCUgUUc- -3' miRNA: 3'- uaUACCGUGA-----ACCGAuGGUGAgAAca -5' |
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27526 | 3' | -50.9 | NC_005879.1 | + | 7212 | 0.66 | 0.771982 |
Target: 5'- ---cGGCACgagcaUUGGCcgACUACUUUUGUu -3' miRNA: 3'- uauaCCGUG-----AACCGa-UGGUGAGAACA- -5' |
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27526 | 3' | -50.9 | NC_005879.1 | + | 6911 | 0.69 | 0.632887 |
Target: 5'- ----cGCGCUUGGgucuuCUGCCACUUUUGUc -3' miRNA: 3'- uauacCGUGAACC-----GAUGGUGAGAACA- -5' |
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27526 | 3' | -50.9 | NC_005879.1 | + | 10145 | 0.73 | 0.418077 |
Target: 5'- gAUGUGGCGCUUGG-UAUCGCaauuccguUCUUGUa -3' miRNA: 3'- -UAUACCGUGAACCgAUGGUG--------AGAACA- -5' |
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27526 | 3' | -50.9 | NC_005879.1 | + | 6807 | 1.07 | 0.001989 |
Target: 5'- gAUAUGGCACUUGGCUACCACUCUUGUa -3' miRNA: 3'- -UAUACCGUGAACCGAUGGUGAGAACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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