miRNA: 3'- auaaAAGA-CGAGGGCGUUGuUCAGGu -5'
miRNA: 3'- auaaaaGACGaGGGCGUUGuuCAGGu -5'
miRNA: 3'- auaaaagaCGAGGGCG--------------UUGUUCAGGU- -5'
miRNA: 3'- aUAAAAGACGaGGGCGUUGU--UCaGGU- -5'
miRNA: 3'- -AUAAAAGACGAGGGCGUUGUUCAGGU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27528 | 3' | -49.1 | NC_005879.1 | + | 14853 | 0.66 | 0.907043 |
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Target: 5'- ---cUUCUuGCUCCUGCAAC-GGUUUg -3' miRNA: 3'- auaaAAGA-CGAGGGCGUUGuUCAGGu -5' |
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| 27528 | 3' | -49.1 | NC_005879.1 | + | 4906 | 0.66 | 0.899678 |
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Target: 5'- -----aUUGC-CCCGCAAUucGUCCu -3' miRNA: 3'- auaaaaGACGaGGGCGUUGuuCAGGu -5' |
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| 27528 | 3' | -49.1 | NC_005879.1 | + | 7498 | 0.71 | 0.622789 |
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Target: 5'- -------cGCUCCCGCucgccaauucaggcGGCAAGUCCGa -3' miRNA: 3'- auaaaagaCGAGGGCG--------------UUGUUCAGGU- -5' |
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| 27528 | 3' | -49.1 | NC_005879.1 | + | 4244 | 0.73 | 0.531575 |
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Target: 5'- gAUUUUCUGCgCUCGCGACAguGGgCCAg -3' miRNA: 3'- aUAAAAGACGaGGGCGUUGU--UCaGGU- -5' |
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| 27528 | 3' | -49.1 | NC_005879.1 | + | 12392 | 1.08 | 0.00268 |
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Target: 5'- uUAUUUUCUGCUCCCGCAACAAGUCCAa -3' miRNA: 3'- -AUAAAAGACGAGGGCGUUGUUCAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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