Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27529 | 3' | -53.2 | NC_005879.1 | + | 39081 | 0.66 | 0.725145 |
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Target: 5'- -cGCaUUUCCGCAugccagaguagccCGAcUUGCAAGGGUa -3' miRNA: 3'- gaCG-AGGGGCGU-------------GCUuAAUGUUCCCG- -5' |
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| 27529 | 3' | -53.2 | NC_005879.1 | + | 37597 | 0.66 | 0.715159 |
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Target: 5'- uUGCUCCuuGUugGAAUgcgGCAGaGuGCc -3' miRNA: 3'- gACGAGGggCGugCUUAa--UGUUcC-CG- -5' |
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| 27529 | 3' | -53.2 | NC_005879.1 | + | 11592 | 0.67 | 0.692723 |
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Target: 5'- uUGCUCCCCGacuucaauucaACGAAU-AUGAGucGGCg -3' miRNA: 3'- gACGAGGGGCg----------UGCUUAaUGUUC--CCG- -5' |
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| 27529 | 3' | -53.2 | NC_005879.1 | + | 1314 | 0.67 | 0.692723 |
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Target: 5'- uUGCgCgCCGCaagaACGAAgcacuaaACAAGGGCg -3' miRNA: 3'- gACGaGgGGCG----UGCUUaa-----UGUUCCCG- -5' |
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| 27529 | 3' | -53.2 | NC_005879.1 | + | 16502 | 0.67 | 0.647189 |
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Target: 5'- -cGCUCUaUCGCAuUGggUUuCGGGGGCg -3' miRNA: 3'- gaCGAGG-GGCGU-GCuuAAuGUUCCCG- -5' |
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| 27529 | 3' | -53.2 | NC_005879.1 | + | 16645 | 0.69 | 0.530452 |
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Target: 5'- -cGCUCCCCG-ACGAGUUGaaaccugagacagcCAAGGcuGCg -3' miRNA: 3'- gaCGAGGGGCgUGCUUAAU--------------GUUCC--CG- -5' |
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| 27529 | 3' | -53.2 | NC_005879.1 | + | 9205 | 0.73 | 0.327549 |
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Target: 5'- aCUGCUUCuaCCGCACGAAUUGCGucgcuuGGUa -3' miRNA: 3'- -GACGAGG--GGCGUGCUUAAUGUuc----CCG- -5' |
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| 27529 | 3' | -53.2 | NC_005879.1 | + | 7237 | 0.74 | 0.273206 |
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Target: 5'- uUGUUCCgaaGCGCGAuuggGCGAGGGCg -3' miRNA: 3'- gACGAGGgg-CGUGCUuaa-UGUUCCCG- -5' |
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| 27529 | 3' | -53.2 | NC_005879.1 | + | 18530 | 1.13 | 0.000556 |
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Target: 5'- gCUGCUCCCCGCACGAAUUACAAGGGCa -3' miRNA: 3'- -GACGAGGGGCGUGCUUAAUGUUCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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