miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27531 5' -42.2 NC_005879.1 + 4568 0.66 0.999885
Target:  5'- aCCACCAcugcgggucAACCAGugcgccguGUUUAcaCGGAAUCAg -3'
miRNA:   3'- -GGUGGU---------UUGGUU--------UAAGUa-GUCUUAGU- -5'
27531 5' -42.2 NC_005879.1 + 3233 0.66 0.999845
Target:  5'- uCCugCAAcGCCGAAUacaCAUCGuGggUCGc -3'
miRNA:   3'- -GGugGUU-UGGUUUAa--GUAGU-CuuAGU- -5'
27531 5' -42.2 NC_005879.1 + 37288 0.66 0.999845
Target:  5'- aCACCGGAC--GAUUCAaaAGAAUCc -3'
miRNA:   3'- gGUGGUUUGguUUAAGUagUCUUAGu -5'
27531 5' -42.2 NC_005879.1 + 31228 0.66 0.999726
Target:  5'- aUACCAcGGCgGAAUU--UCAGAGUCAg -3'
miRNA:   3'- gGUGGU-UUGgUUUAAguAGUCUUAGU- -5'
27531 5' -42.2 NC_005879.1 + 26720 0.67 0.999532
Target:  5'- aCCGCCGAcACCAAAcca---AGAAUCAa -3'
miRNA:   3'- -GGUGGUU-UGGUUUaaguagUCUUAGU- -5'
27531 5' -42.2 NC_005879.1 + 19711 0.68 0.998465
Target:  5'- gCGCCGAACggagaaAAAUUCAUCAagGAAUUg -3'
miRNA:   3'- gGUGGUUUGg-----UUUAAGUAGU--CUUAGu -5'
27531 5' -42.2 NC_005879.1 + 1713 0.69 0.99714
Target:  5'- gCACCAa--CAAGcUUAUCGGAGUCGc -3'
miRNA:   3'- gGUGGUuugGUUUaAGUAGUCUUAGU- -5'
27531 5' -42.2 NC_005879.1 + 12245 0.71 0.982474
Target:  5'- cCCACgAAACUAAuagggUCAUUAGGAUUg -3'
miRNA:   3'- -GGUGgUUUGGUUua---AGUAGUCUUAGu -5'
27531 5' -42.2 NC_005879.1 + 13505 0.71 0.982474
Target:  5'- -gGCCAAGCCGAagcggauuuggGUUCGUCAGGc--- -3'
miRNA:   3'- ggUGGUUUGGUU-----------UAAGUAGUCUuagu -5'
27531 5' -42.2 NC_005879.1 + 26196 0.75 0.912882
Target:  5'- aCCGCCAAccauucaucguACuCAAGcUCGUCGGggUCGa -3'
miRNA:   3'- -GGUGGUU-----------UG-GUUUaAGUAGUCuuAGU- -5'
27531 5' -42.2 NC_005879.1 + 22685 1.14 0.00908
Target:  5'- cCCACCAAACCAAAUUCAUCAGAAUCAa -3'
miRNA:   3'- -GGUGGUUUGGUUUAAGUAGUCUUAGU- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.