miRNA display CGI


Results 1 - 12 of 12 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27541 5' -43.7 NC_005881.1 + 52480 0.66 1
Target:  5'- uUUAUAUCCuccgaGGACCGauUCCAAGAaguuacUUUCAc -3'
miRNA:   3'- -AAUGUAGG-----UCUGGU--AGGUUUU------AAAGU- -5'
27541 5' -43.7 NC_005881.1 + 2334 0.67 1
Target:  5'- -cGCggCCAGAUCGUCCAuucgUUUAu -3'
miRNA:   3'- aaUGuaGGUCUGGUAGGUuuuaAAGU- -5'
27541 5' -43.7 NC_005881.1 + 116053 0.66 1
Target:  5'- -aGCAUCU--ACCcUCUAAAAUUUCAg -3'
miRNA:   3'- aaUGUAGGucUGGuAGGUUUUAAAGU- -5'
27541 5' -43.7 NC_005881.1 + 163436 0.67 1
Target:  5'- -aAC-UCCAG-CCAUCCAcauGUUUCu -3'
miRNA:   3'- aaUGuAGGUCuGGUAGGUuu-UAAAGu -5'
27541 5' -43.7 NC_005881.1 + 56655 0.67 1
Target:  5'- --cCAUCCAcACCAUCCAAua--UCAu -3'
miRNA:   3'- aauGUAGGUcUGGUAGGUUuuaaAGU- -5'
27541 5' -43.7 NC_005881.1 + 180702 0.67 1
Target:  5'- -cGCggCCAGAUCGUCCAuucgUUUAu -3'
miRNA:   3'- aaUGuaGGUCUGGUAGGUuuuaAAGU- -5'
27541 5' -43.7 NC_005881.1 + 59150 0.67 0.999999
Target:  5'- -aAUGUCUcuuauuGGACCuggCCAAAAUUUCAa -3'
miRNA:   3'- aaUGUAGG------UCUGGua-GGUUUUAAAGU- -5'
27541 5' -43.7 NC_005881.1 + 161585 0.68 0.999997
Target:  5'- cUGCuAUCCAcGCUAUCCAuGAUUUUAa -3'
miRNA:   3'- aAUG-UAGGUcUGGUAGGUuUUAAAGU- -5'
27541 5' -43.7 NC_005881.1 + 24235 0.69 0.999985
Target:  5'- -aACAUCCuuGCCAUCCAAAc----- -3'
miRNA:   3'- aaUGUAGGucUGGUAGGUUUuaaagu -5'
27541 5' -43.7 NC_005881.1 + 113200 0.7 0.999948
Target:  5'- aUACGUCCAGACCAgagUUAAAucuauUUUUAg -3'
miRNA:   3'- aAUGUAGGUCUGGUa--GGUUUu----AAAGU- -5'
27541 5' -43.7 NC_005881.1 + 80609 0.74 0.996013
Target:  5'- aUUACAUCCAGggcuuugGCCAUCCuuAAUUUa- -3'
miRNA:   3'- -AAUGUAGGUC-------UGGUAGGuuUUAAAgu -5'
27541 5' -43.7 NC_005881.1 + 113392 1.08 0.061993
Target:  5'- aUUACAUCCAGACCAUCCAAAAUUUCAu -3'
miRNA:   3'- -AAUGUAGGUCUGGUAGGUUUUAAAGU- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.