Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27543 | 3' | -46.9 | NC_005881.1 | + | 8552 | 0.66 | 0.999964 |
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Target: 5'- uCAUUGGCgUGAUguugucaagcAGGAUGAUGaGGUUa- -3' miRNA: 3'- -GUAACCG-ACUA----------UCCUAUUAC-CUAGgu -5' |
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| 27543 | 3' | -46.9 | NC_005881.1 | + | 94309 | 0.66 | 0.999963 |
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Target: 5'- ---aGGCUG-UGGGAUGuauagccGUGGAgcgugCCAa -3' miRNA: 3'- guaaCCGACuAUCCUAU-------UACCUa----GGU- -5' |
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| 27543 | 3' | -46.9 | NC_005881.1 | + | 175322 | 0.67 | 0.999916 |
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Target: 5'- gAUUuGCUGG-AGGucuUAAUGGAUCCu -3' miRNA: 3'- gUAAcCGACUaUCCu--AUUACCUAGGu -5' |
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| 27543 | 3' | -46.9 | NC_005881.1 | + | 7715 | 0.67 | 0.999916 |
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Target: 5'- gAUUuGCUGG-AGGucuUAAUGGAUCCu -3' miRNA: 3'- gUAAcCGACUaUCCu--AUUACCUAGGu -5' |
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| 27543 | 3' | -46.9 | NC_005881.1 | + | 139986 | 0.73 | 0.97912 |
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Target: 5'- uUAUUGGCUGGgcaGGGAuucUAuuaauagauuuugGUGGAUCCAu -3' miRNA: 3'- -GUAACCGACUa--UCCU---AU-------------UACCUAGGU- -5' |
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| 27543 | 3' | -46.9 | NC_005881.1 | + | 140256 | 1.09 | 0.026534 |
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Target: 5'- uCAUUGGCUGAUAGGAUAAUGGAUCCAc -3' miRNA: 3'- -GUAACCGACUAUCCUAUUACCUAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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