Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27543 | 5' | -49.7 | NC_005881.1 | + | 5698 | 0.66 | 0.997889 |
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Target: 5'- --gUAAUAuGAuUUCCUGCCCAGUa-- -3' miRNA: 3'- auaAUUAU-CUuAGGGACGGGUCGguu -5' |
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| 27543 | 5' | -49.7 | NC_005881.1 | + | 60077 | 0.66 | 0.997889 |
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Target: 5'- --aUGGUAGGcucuUCCCUGCC--GCCAAu -3' miRNA: 3'- auaAUUAUCUu---AGGGACGGguCGGUU- -5' |
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| 27543 | 5' | -49.7 | NC_005881.1 | + | 177339 | 0.66 | 0.997889 |
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Target: 5'- --gUAAUAuGAuUUCCUGCCCAGUa-- -3' miRNA: 3'- auaAUUAU-CUuAGGGACGGGUCGguu -5' |
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| 27543 | 5' | -49.7 | NC_005881.1 | + | 140952 | 0.66 | 0.996996 |
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Target: 5'- -cUUGAUAGAGUCCggcaUUGCCacaGGCCc- -3' miRNA: 3'- auAAUUAUCUUAGG----GACGGg--UCGGuu -5' |
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| 27543 | 5' | -49.7 | NC_005881.1 | + | 163561 | 0.66 | 0.996444 |
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Target: 5'- --gUAAUAcca-CCCUGCCuCAGCCAu -3' miRNA: 3'- auaAUUAUcuuaGGGACGG-GUCGGUu -5' |
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| 27543 | 5' | -49.7 | NC_005881.1 | + | 78858 | 0.67 | 0.993344 |
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Target: 5'- ------gAGAAaacgaucuacuUCCC-GCCCAGCCAAu -3' miRNA: 3'- auaauuaUCUU-----------AGGGaCGGGUCGGUU- -5' |
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| 27543 | 5' | -49.7 | NC_005881.1 | + | 140222 | 1.05 | 0.020673 |
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Target: 5'- cUAUUAAUAGAAUCCCUGCCCAGCCAAu -3' miRNA: 3'- -AUAAUUAUCUUAGGGACGGGUCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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