Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27544 | 5' | -56.8 | NC_005881.1 | + | 135045 | 0.66 | 0.903355 |
Target: 5'- aCACCugCACcauCCAUCGGUAG-GGCg -3' miRNA: 3'- -GUGGugGUGuu-GGUGGUCGUCgUCGa -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 24058 | 0.66 | 0.903355 |
Target: 5'- aCACUACCAaAGCCAUCuaaacagaAGCAGgAGCc -3' miRNA: 3'- -GUGGUGGUgUUGGUGG--------UCGUCgUCGa -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 146862 | 0.66 | 0.897012 |
Target: 5'- aGCCACCuccaccaacaACAACaucagCACCAGCAGUugauguGGCa -3' miRNA: 3'- gUGGUGG----------UGUUG-----GUGGUCGUCG------UCGa -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 125016 | 0.66 | 0.897012 |
Target: 5'- uCACCuCCAUAAUCugCGGUuGCGGUc -3' miRNA: 3'- -GUGGuGGUGUUGGugGUCGuCGUCGa -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 144725 | 0.66 | 0.897012 |
Target: 5'- --aCAuCCGCAACCACCucuuuGCGGaUAGCg -3' miRNA: 3'- gugGU-GGUGUUGGUGGu----CGUC-GUCGa -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 197535 | 0.66 | 0.890442 |
Target: 5'- aCugUAUauaaGCAGCCGCCAGU-GUAGCa -3' miRNA: 3'- -GugGUGg---UGUUGGUGGUCGuCGUCGa -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 179373 | 0.67 | 0.875922 |
Target: 5'- gACCugUGCccguuaGACUgggaaagaggcguGCCAGCAGCGGCg -3' miRNA: 3'- gUGGugGUG------UUGG-------------UGGUCGUCGUCGa -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 3664 | 0.67 | 0.875922 |
Target: 5'- gACCugUGCccguuaGACUgggaaagaggcguGCCAGCAGCGGCg -3' miRNA: 3'- gUGGugGUG------UUGG-------------UGGUCGUCGUCGa -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 98756 | 0.67 | 0.868674 |
Target: 5'- cUACCACCACAgaaGCCACUaccacaaGGguGaCAGUUc -3' miRNA: 3'- -GUGGUGGUGU---UGGUGG-------UCguC-GUCGA- -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 181974 | 0.67 | 0.854331 |
Target: 5'- aCGCUACCuAguGCCagagaACUGGCGGCGGCa -3' miRNA: 3'- -GUGGUGG-UguUGG-----UGGUCGUCGUCGa -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 1062 | 0.67 | 0.854331 |
Target: 5'- aCGCUACCuAguGCCagagaACUGGCGGCGGCa -3' miRNA: 3'- -GUGGUGG-UguUGG-----UGGUCGUCGUCGa -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 96652 | 0.67 | 0.846493 |
Target: 5'- cCACCACCACAACCACaccuGCGcCAa-- -3' miRNA: 3'- -GUGGUGGUGUUGGUGgu--CGUcGUcga -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 134786 | 0.67 | 0.8352 |
Target: 5'- aACCACCACAcaagcuccuccuccACCuaucguuauuaauACCA-CAGCAGCa -3' miRNA: 3'- gUGGUGGUGU--------------UGG-------------UGGUcGUCGUCGa -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 158529 | 0.68 | 0.804576 |
Target: 5'- uUACCACCucugACAacACCGCCAGUggAGUGGUUg -3' miRNA: 3'- -GUGGUGG----UGU--UGGUGGUCG--UCGUCGA- -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 171427 | 0.69 | 0.78668 |
Target: 5'- gGCCACCACAAUaaacaCAGCGuCAGCa -3' miRNA: 3'- gUGGUGGUGUUGgug--GUCGUcGUCGa -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 146812 | 0.69 | 0.739702 |
Target: 5'- gCACCACCaACGuuuggguuuaacACCACCAGUAcUAGCa -3' miRNA: 3'- -GUGGUGG-UGU------------UGGUGGUCGUcGUCGa -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 93126 | 0.72 | 0.608498 |
Target: 5'- aCAUCACCaAUGACCAaaugguuCCAGCAcGCAGCg -3' miRNA: 3'- -GUGGUGG-UGUUGGU-------GGUCGU-CGUCGa -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 506 | 0.73 | 0.539408 |
Target: 5'- uCACCACCGcCAACCAauUCAGCAGUgacAGUUa -3' miRNA: 3'- -GUGGUGGU-GUUGGU--GGUCGUCG---UCGA- -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 182530 | 0.73 | 0.539408 |
Target: 5'- uCACCACCGcCAACCAauUCAGCAGUgacAGUUa -3' miRNA: 3'- -GUGGUGGU-GUUGGU--GGUCGUCG---UCGA- -5' |
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27544 | 5' | -56.8 | NC_005881.1 | + | 1755 | 0.76 | 0.360158 |
Target: 5'- uCGCCGCCAU-ACUACagAGCAGCAGCg -3' miRNA: 3'- -GUGGUGGUGuUGGUGg-UCGUCGUCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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