Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27545 | 5' | -46 | NC_005881.1 | + | 43830 | 0.66 | 0.999998 |
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Target: 5'- uCAAAGAuGGgcgUUUC-AGAGACCaUGAu -3' miRNA: 3'- -GUUUCU-CCa--AAAGuUCUCUGGcACUc -5' |
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| 27545 | 5' | -46 | NC_005881.1 | + | 107829 | 0.66 | 0.999997 |
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Target: 5'- aGAAGAGGg----AGGAGAaggCGUGAGg -3' miRNA: 3'- gUUUCUCCaaaagUUCUCUg--GCACUC- -5' |
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| 27545 | 5' | -46 | NC_005881.1 | + | 35077 | 0.68 | 0.999879 |
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Target: 5'- uCAAAGAGuauGUUUgUCAGGaAGAUCGUGAa -3' miRNA: 3'- -GUUUCUC---CAAA-AGUUC-UCUGGCACUc -5' |
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| 27545 | 5' | -46 | NC_005881.1 | + | 202814 | 0.69 | 0.999843 |
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Target: 5'- gAGAGAGG---UCAAGAGuuuaCGUGGGu -3' miRNA: 3'- gUUUCUCCaaaAGUUCUCug--GCACUC- -5' |
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| 27545 | 5' | -46 | NC_005881.1 | + | 188962 | 0.69 | 0.999843 |
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Target: 5'- gAGAGAGG---UCAAGAGuuuaCGUGGGu -3' miRNA: 3'- gUUUCUCCaaaAGUUCUCug--GCACUC- -5' |
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| 27545 | 5' | -46 | NC_005881.1 | + | 207171 | 0.7 | 0.999203 |
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Target: 5'- -uAGGAGGggUUUAGGGGGUgGUGAGg -3' miRNA: 3'- guUUCUCCaaAAGUUCUCUGgCACUC- -5' |
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| 27545 | 5' | -46 | NC_005881.1 | + | 184605 | 0.7 | 0.999203 |
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Target: 5'- -uAGGAGGggUUUAGGGGGUgGUGAGg -3' miRNA: 3'- guUUCUCCaaAAGUUCUCUGgCACUC- -5' |
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| 27545 | 5' | -46 | NC_005881.1 | + | 76060 | 0.71 | 0.998547 |
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Target: 5'- --uGGAGGgaUUCGacAGGGAUCGUGAu -3' miRNA: 3'- guuUCUCCaaAAGU--UCUCUGGCACUc -5' |
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| 27545 | 5' | -46 | NC_005881.1 | + | 120055 | 0.71 | 0.998246 |
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Target: 5'- aCAAAGAGGUUgc--AGAGGCaaugGUGAGu -3' miRNA: 3'- -GUUUCUCCAAaaguUCUCUGg---CACUC- -5' |
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| 27545 | 5' | -46 | NC_005881.1 | + | 158651 | 1.11 | 0.027653 |
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Target: 5'- cCAAAGAGGUUUUCAAGAGACCGUGAGg -3' miRNA: 3'- -GUUUCUCCAAAAGUUCUCUGGCACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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