Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27547 | 5' | -51 | NC_005881.1 | + | 33399 | 0.66 | 0.995601 |
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Target: 5'- uUGG-UGACCAcuuacAUGGcuGGAGUCAGAGa -3' miRNA: 3'- -AUCuGCUGGU-----UACCuuUCUCGGUCUU- -5' |
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| 27547 | 5' | -51 | NC_005881.1 | + | 1085 | 0.66 | 0.993483 |
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Target: 5'- cGGACGAUUAcAUGGGAcaggucacgcuaccuAGuGCCAGAGa -3' miRNA: 3'- aUCUGCUGGU-UACCUU---------------UCuCGGUCUU- -5' |
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| 27547 | 5' | -51 | NC_005881.1 | + | 181951 | 0.66 | 0.993483 |
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Target: 5'- cGGACGAUUAcAUGGGAcaggucacgcuaccuAGuGCCAGAGa -3' miRNA: 3'- aUCUGCUGGU-UACCUU---------------UCuCGGUCUU- -5' |
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| 27547 | 5' | -51 | NC_005881.1 | + | 40088 | 0.66 | 0.993095 |
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Target: 5'- aAGACuacAUCAAUGGAAuugagGGAGCCAuGAAu -3' miRNA: 3'- aUCUGc--UGGUUACCUU-----UCUCGGU-CUU- -5' |
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| 27547 | 5' | -51 | NC_005881.1 | + | 81565 | 0.67 | 0.98651 |
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Target: 5'- cAGAUguugGACCGGUGGGAucuGCCAGGc -3' miRNA: 3'- aUCUG----CUGGUUACCUUucuCGGUCUu -5' |
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| 27547 | 5' | -51 | NC_005881.1 | + | 187367 | 0.7 | 0.948019 |
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Target: 5'- -cGGCGAUUggUGGGAGGAGgUGGAGg -3' miRNA: 3'- auCUGCUGGuuACCUUUCUCgGUCUU- -5' |
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| 27547 | 5' | -51 | NC_005881.1 | + | 155304 | 0.7 | 0.948019 |
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Target: 5'- aUGGuAC-ACCAGUaGAGGGAGCCAGAGg -3' miRNA: 3'- -AUC-UGcUGGUUAcCUUUCUCGGUCUU- -5' |
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| 27547 | 5' | -51 | NC_005881.1 | + | 204410 | 0.7 | 0.948019 |
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Target: 5'- -cGGCGAUUggUGGGAGGAGgUGGAGg -3' miRNA: 3'- auCUGCUGGuuACCUUUCUCgGUCUU- -5' |
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| 27547 | 5' | -51 | NC_005881.1 | + | 187564 | 1.05 | 0.016117 |
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Target: 5'- uUAGACGACCAAUGGAAAGAGCCAGAAa -3' miRNA: 3'- -AUCUGCUGGUUACCUUUCUCGGUCUU- -5' |
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| 27547 | 5' | -51 | NC_005881.1 | + | 204212 | 1.05 | 0.016117 |
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Target: 5'- uUAGACGACCAAUGGAAAGAGCCAGAAa -3' miRNA: 3'- -AUCUGCUGGUUACCUUUCUCGGUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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