Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2755 | 3' | -49.9 | NC_001491.2 | + | 55185 | 0.66 | 0.995089 |
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Target: 5'- aGACcGCGGAg---ACAgaGCGCCCa- -3' miRNA: 3'- gCUGuCGCCUaaaaUGUa-CGUGGGac -5' |
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| 2755 | 3' | -49.9 | NC_001491.2 | + | 55073 | 0.66 | 0.995089 |
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Target: 5'- gCGAC-GCGGug--UACAcguggGCACCUUGg -3' miRNA: 3'- -GCUGuCGCCuaaaAUGUa----CGUGGGAC- -5' |
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| 2755 | 3' | -49.9 | NC_001491.2 | + | 37604 | 0.66 | 0.994278 |
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Target: 5'- gCGGCAGCGGGU---GCca-CACCCUc -3' miRNA: 3'- -GCUGUCGCCUAaaaUGuacGUGGGAc -5' |
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| 2755 | 3' | -49.9 | NC_001491.2 | + | 146540 | 0.67 | 0.993362 |
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Target: 5'- uGAaGGCGGAcg--GCcuggGCGCCCUGg -3' miRNA: 3'- gCUgUCGCCUaaaaUGua--CGUGGGAC- -5' |
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| 2755 | 3' | -49.9 | NC_001491.2 | + | 143225 | 0.67 | 0.993265 |
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Target: 5'- aGGCGGUGGcgcuGUuucaaaacacgguUUUACAUGCGCCUUc -3' miRNA: 3'- gCUGUCGCC----UA-------------AAAUGUACGUGGGAc -5' |
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| 2755 | 3' | -49.9 | NC_001491.2 | + | 123036 | 0.68 | 0.983238 |
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Target: 5'- gCGGCAGCGaGAcc--GCGUGCGCCg-- -3' miRNA: 3'- -GCUGUCGC-CUaaaaUGUACGUGGgac -5' |
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| 2755 | 3' | -49.9 | NC_001491.2 | + | 121823 | 0.69 | 0.976388 |
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Target: 5'- uCGAguGUGGGgcgcgugUGCGUGUACCCc- -3' miRNA: 3'- -GCUguCGCCUaaa----AUGUACGUGGGac -5' |
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| 2755 | 3' | -49.9 | NC_001491.2 | + | 37107 | 0.71 | 0.928605 |
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Target: 5'- uGACgAGCGGGUaUUUGCGUGCucugaaACCCa- -3' miRNA: 3'- gCUG-UCGCCUA-AAAUGUACG------UGGGac -5' |
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| 2755 | 3' | -49.9 | NC_001491.2 | + | 137095 | 0.71 | 0.917083 |
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Target: 5'- uGAUGGgGGAg---ACggGCACCCUGa -3' miRNA: 3'- gCUGUCgCCUaaaaUGuaCGUGGGAC- -5' |
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| 2755 | 3' | -49.9 | NC_001491.2 | + | 117435 | 0.73 | 0.876324 |
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Target: 5'- cCGGCGGCGGcuu---CGUGCGCCCc- -3' miRNA: 3'- -GCUGUCGCCuaaaauGUACGUGGGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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