miRNA display CGI


Results 1 - 10 of 10 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27550 3' -56.3 NC_005881.1 + 109568 0.66 0.920943
Target:  5'- cUGGGAaaacauuaucAUCAUCUUCAUCAUCugCGGa -3'
miRNA:   3'- uACCCU----------UAGUGGAGGUGGUGGugGUU- -5'
27550 3' -56.3 NC_005881.1 + 85260 0.66 0.920943
Target:  5'- uUGcGGA---GCCcCCACCACCACCc- -3'
miRNA:   3'- uAC-CCUuagUGGaGGUGGUGGUGGuu -5'
27550 3' -56.3 NC_005881.1 + 54352 0.66 0.90313
Target:  5'- cUGGuGAAaaACCUCCGCCAaagaacaaaaagUCACCGAa -3'
miRNA:   3'- uACC-CUUagUGGAGGUGGU------------GGUGGUU- -5'
27550 3' -56.3 NC_005881.1 + 3466 0.67 0.868824
Target:  5'- gGUGGGAcauacaauUCACCUCUaauuGCCGCUaugGCCAu -3'
miRNA:   3'- -UACCCUu-------AGUGGAGG----UGGUGG---UGGUu -5'
27550 3' -56.3 NC_005881.1 + 179570 0.67 0.868824
Target:  5'- gGUGGGAcauacaauUCACCUCUaauuGCCGCUaugGCCAu -3'
miRNA:   3'- -UACCCUu-------AGUGGAGG----UGGUGG---UGGUu -5'
27550 3' -56.3 NC_005881.1 + 124632 0.68 0.845654
Target:  5'- -aGaGGAAgaaccaaCACCUCCACCACCAgUg- -3'
miRNA:   3'- uaC-CCUUa------GUGGAGGUGGUGGUgGuu -5'
27550 3' -56.3 NC_005881.1 + 28659 0.68 0.845654
Target:  5'- uUGGGuaaauauuuguuGAUCGCCUCCACaauaggucuGCCGCUAAu -3'
miRNA:   3'- uACCC------------UUAGUGGAGGUGg--------UGGUGGUU- -5'
27550 3' -56.3 NC_005881.1 + 154178 0.7 0.720943
Target:  5'- -aGGGuggccgcggcagcacCACCUCCACCACCACa-- -3'
miRNA:   3'- uaCCCuua------------GUGGAGGUGGUGGUGguu -5'
27550 3' -56.3 NC_005881.1 + 134706 0.71 0.677748
Target:  5'- ---cGAuUCACCaCCACCGCCACCAAc -3'
miRNA:   3'- uaccCUuAGUGGaGGUGGUGGUGGUU- -5'
27550 3' -56.3 NC_005881.1 + 160037 1.04 0.005875
Target:  5'- gAUGGGAAUCACCUCCACCACCACCAAa -3'
miRNA:   3'- -UACCCUUAGUGGAGGUGGUGGUGGUU- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.