miRNA display CGI

Results 1 - 10 of 10 are showing below:
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ID	Location	Perfect MFE.*	RefSeq Acc.	strand	Start Position	R_P_ratio#	P value
	Predicted miRNA align pattern
27550	3'	-56.3	NC_005881.1	+	109568	0.66	0.920943
	Target:  5'- cUGGGAaaacauuaucAUCAUCUUCAUCAUCugCGGa -3' miRNA:   3'- uACCCU----------UAGUGGAGGUGGUGGugGUU- -5'
27550	3'	-56.3	NC_005881.1	+	85260	0.66	0.920943
	Target:  5'- uUGcGGA---GCCcCCACCACCACCc- -3' miRNA:   3'- uAC-CCUuagUGGaGGUGGUGGUGGuu -5'
27550	3'	-56.3	NC_005881.1	+	54352	0.66	0.90313
	Target:  5'- cUGGuGAAaaACCUCCGCCAaagaacaaaaagUCACCGAa -3' miRNA:   3'- uACC-CUUagUGGAGGUGGU------------GGUGGUU- -5'
27550	3'	-56.3	NC_005881.1	+	3466	0.67	0.868824
	Target:  5'- gGUGGGAcauacaauUCACCUCUaauuGCCGCUaugGCCAu -3' miRNA:   3'- -UACCCUu-------AGUGGAGG----UGGUGG---UGGUu -5'
27550	3'	-56.3	NC_005881.1	+	179570	0.67	0.868824
	Target:  5'- gGUGGGAcauacaauUCACCUCUaauuGCCGCUaugGCCAu -3' miRNA:   3'- -UACCCUu-------AGUGGAGG----UGGUGG---UGGUu -5'
27550	3'	-56.3	NC_005881.1	+	28659	0.68	0.845654
	Target:  5'- uUGGGuaaauauuuguuGAUCGCCUCCACaauaggucuGCCGCUAAu -3' miRNA:   3'- uACCC------------UUAGUGGAGGUGg--------UGGUGGUU- -5'
27550	3'	-56.3	NC_005881.1	+	124632	0.68	0.845654
	Target:  5'- -aGaGGAAgaaccaaCACCUCCACCACCAgUg- -3' miRNA:   3'- uaC-CCUUa------GUGGAGGUGGUGGUgGuu -5'
27550	3'	-56.3	NC_005881.1	+	154178	0.7	0.720943
	Target:  5'- -aGGGuggccgcggcagcacCACCUCCACCACCACa-- -3' miRNA:   3'- uaCCCuua------------GUGGAGGUGGUGGUGguu -5'
27550	3'	-56.3	NC_005881.1	+	134706	0.71	0.677748
	Target:  5'- ---cGAuUCACCaCCACCGCCACCAAc -3' miRNA:   3'- uaccCUuAGUGGaGGUGGUGGUGGUU- -5'
27550	3'	-56.3	NC_005881.1	+	160037	1.04	0.005875
	Target:  5'- gAUGGGAAUCACCUCCACCACCACCAAa -3' miRNA:   3'- -UACCCUUAGUGGAGGUGGUGGUGGUU- -5'

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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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