Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27550 | 5' | -44.3 | NC_005881.1 | + | 202996 | 0.66 | 1 |
Target: 5'- gCCCUGUuugcaauauggaaUGGCCAa--UGAGAAAGGa- -3' miRNA: 3'- -GGGAUA-------------ACCGGUacaACUCUUUUUgg -5' |
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27550 | 5' | -44.3 | NC_005881.1 | + | 188780 | 0.66 | 1 |
Target: 5'- gCCCUGUuugcaauauggaaUGGCCAa--UGAGAAAGGa- -3' miRNA: 3'- -GGGAUA-------------ACCGGUacaACUCUUUUUgg -5' |
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27550 | 5' | -44.3 | NC_005881.1 | + | 168159 | 0.68 | 0.999999 |
Target: 5'- -----aUGGCCAUGUcGGGAAAuugguucaagaaacuAACCa -3' miRNA: 3'- gggauaACCGGUACAaCUCUUU---------------UUGG- -5' |
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27550 | 5' | -44.3 | NC_005881.1 | + | 18890 | 0.67 | 0.999999 |
Target: 5'- gCCaaggUGUUGGCCAUGaUGAu---AACCa -3' miRNA: 3'- -GGg---AUAACCGGUACaACUcuuuUUGG- -5' |
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27550 | 5' | -44.3 | NC_005881.1 | + | 114884 | 0.68 | 0.999998 |
Target: 5'- aCCCggagAUUGGU--UGUUGGGAGAuuCUc -3' miRNA: 3'- -GGGa---UAACCGguACAACUCUUUuuGG- -5' |
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27550 | 5' | -44.3 | NC_005881.1 | + | 163594 | 0.69 | 0.999994 |
Target: 5'- aUCUUGUUucauuuguuucGGCCAUGU--AGAAAAACUa -3' miRNA: 3'- -GGGAUAA-----------CCGGUACAacUCUUUUUGG- -5' |
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27550 | 5' | -44.3 | NC_005881.1 | + | 60313 | 0.69 | 0.999989 |
Target: 5'- uUCUAUUGGCgGca--GGGAAGAGCCu -3' miRNA: 3'- gGGAUAACCGgUacaaCUCUUUUUGG- -5' |
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27550 | 5' | -44.3 | NC_005881.1 | + | 7073 | 0.69 | 0.999978 |
Target: 5'- uCCCg---GGCUuuuuaauucUGUUGAGAuuGACCu -3' miRNA: 3'- -GGGauaaCCGGu--------ACAACUCUuuUUGG- -5' |
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27550 | 5' | -44.3 | NC_005881.1 | + | 175963 | 0.69 | 0.999978 |
Target: 5'- uCCCg---GGCUuuuuaauucUGUUGAGAuuGACCu -3' miRNA: 3'- -GGGauaaCCGGu--------ACAACUCUuuUUGG- -5' |
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27550 | 5' | -44.3 | NC_005881.1 | + | 106336 | 0.69 | 0.999978 |
Target: 5'- cCUCUAaagccacGGCCAUGUUGAaAAAGGCa -3' miRNA: 3'- -GGGAUaa-----CCGGUACAACUcUUUUUGg -5' |
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27550 | 5' | -44.3 | NC_005881.1 | + | 27401 | 0.7 | 0.99997 |
Target: 5'- cUCCUAUUGGUacaagauugcuUccGUUGAGAAGuGACCu -3' miRNA: 3'- -GGGAUAACCG-----------GuaCAACUCUUU-UUGG- -5' |
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27550 | 5' | -44.3 | NC_005881.1 | + | 198216 | 0.74 | 0.997833 |
Target: 5'- aCUCUAgUGGCCAa--UGAGAGAAGCg -3' miRNA: 3'- -GGGAUaACCGGUacaACUCUUUUUGg -5' |
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27550 | 5' | -44.3 | NC_005881.1 | + | 193560 | 0.74 | 0.997833 |
Target: 5'- aCUCUAgUGGCCAa--UGAGAGAAGCg -3' miRNA: 3'- -GGGAUaACCGGUacaACUCUUUUUGg -5' |
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27550 | 5' | -44.3 | NC_005881.1 | + | 169697 | 0.82 | 0.887577 |
Target: 5'- -aCUGUgGGCUuuUGUUGAGAGAAGCCg -3' miRNA: 3'- ggGAUAaCCGGu-ACAACUCUUUUUGG- -5' |
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27550 | 5' | -44.3 | NC_005881.1 | + | 160071 | 1.18 | 0.019335 |
Target: 5'- uCCCUAUUGGCCAUGUUGAGAAAAACCg -3' miRNA: 3'- -GGGAUAACCGGUACAACUCUUUUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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