Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27551 | 3' | -48.8 | NC_005881.1 | + | 124679 | 0.66 | 0.99963 |
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Target: 5'- aGGUGGGGGAGGUg-GAGGa------- -3' miRNA: 3'- -CUACCCCCUCUAaaCUCCgcuaaaac -5' |
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| 27551 | 3' | -48.8 | NC_005881.1 | + | 2761 | 0.71 | 0.978412 |
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Target: 5'- -uUGGGGGGGGgggGGGGgGGUgUUUGg -3' miRNA: 3'- cuACCCCCUCUaaaCUCCgCUA-AAAC- -5' |
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| 27551 | 3' | -48.8 | NC_005881.1 | + | 180276 | 0.71 | 0.978412 |
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Target: 5'- -uUGGGGGGGGgggGGGGgGGUgUUUGg -3' miRNA: 3'- cuACCCCCUCUaaaCUCCgCUA-AAAC- -5' |
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| 27551 | 3' | -48.8 | NC_005881.1 | + | 182799 | 0.78 | 0.741965 |
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Target: 5'- gGAUGGGGGAGGUguugggGAGGUGGg---- -3' miRNA: 3'- -CUACCCCCUCUAaa----CUCCGCUaaaac -5' |
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| 27551 | 3' | -48.8 | NC_005881.1 | + | 237 | 0.78 | 0.741965 |
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Target: 5'- gGAUGGGGGAGGUguugggGAGGUGGg---- -3' miRNA: 3'- -CUACCCCCUCUAaa----CUCCGCUaaaac -5' |
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| 27551 | 3' | -48.8 | NC_005881.1 | + | 184298 | 1.08 | 0.020268 |
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Target: 5'- gGAUGGGGGAGAUUUGAGGCGAUUUUGc -3' miRNA: 3'- -CUACCCCCUCUAAACUCCGCUAAAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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