Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27551 | 5' | -59.5 | NC_005881.1 | + | 134721 | 0.66 | 0.851393 |
Target: 5'- gCCaCCAACUCCaCUC-AGGCgCCUCCUCc -3' miRNA: 3'- -GG-GGUUGGGGgGAGaUUUG-GGGGGAG- -5' |
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27551 | 5' | -59.5 | NC_005881.1 | + | 2987 | 0.66 | 0.843786 |
Target: 5'- aCCCCccCCCCCC------CCCCCCa- -3' miRNA: 3'- -GGGGuuGGGGGGagauuuGGGGGGag -5' |
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27551 | 5' | -59.5 | NC_005881.1 | + | 180049 | 0.66 | 0.843786 |
Target: 5'- aCCCCccCCCCCC------CCCCCCa- -3' miRNA: 3'- -GGGGuuGGGGGGagauuuGGGGGGag -5' |
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27551 | 5' | -59.5 | NC_005881.1 | + | 193405 | 0.66 | 0.828052 |
Target: 5'- gCUCUGGCUCCaCCUC-AGGCUCCuCCUCc -3' miRNA: 3'- -GGGGUUGGGG-GGAGaUUUGGGG-GGAG- -5' |
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27551 | 5' | -59.5 | NC_005881.1 | + | 198372 | 0.66 | 0.828052 |
Target: 5'- gCUCUGGCUCCaCCUC-AGGCUCCuCCUCc -3' miRNA: 3'- -GGGGUUGGGG-GGAGaUUUGGGG-GGAG- -5' |
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27551 | 5' | -59.5 | NC_005881.1 | + | 188726 | 0.66 | 0.819939 |
Target: 5'- -aCCAACCCacguaaaCUCUuGACCUCUCUCu -3' miRNA: 3'- ggGGUUGGGgg-----GAGAuUUGGGGGGAG- -5' |
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27551 | 5' | -59.5 | NC_005881.1 | + | 203050 | 0.66 | 0.819939 |
Target: 5'- -aCCAACCCacguaaaCUCUuGACCUCUCUCu -3' miRNA: 3'- ggGGUUGGGgg-----GAGAuUUGGGGGGAG- -5' |
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27551 | 5' | -59.5 | NC_005881.1 | + | 28291 | 0.68 | 0.759209 |
Target: 5'- aCCC-AUCCCauaaUGAACCCUCCUCa -3' miRNA: 3'- gGGGuUGGGGggagAUUUGGGGGGAG- -5' |
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27551 | 5' | -59.5 | NC_005881.1 | + | 125685 | 0.68 | 0.722085 |
Target: 5'- cCCCCAACUCCCUcccCUAAcUCCUCCa- -3' miRNA: 3'- -GGGGUUGGGGGGa--GAUUuGGGGGGag -5' |
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27551 | 5' | -59.5 | NC_005881.1 | + | 22 | 0.74 | 0.396994 |
Target: 5'- cCCCCAuccuCCCCaCCUCcuccCCCUCCUCu -3' miRNA: 3'- -GGGGUu---GGGG-GGAGauuuGGGGGGAG- -5' |
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27551 | 5' | -59.5 | NC_005881.1 | + | 183014 | 0.74 | 0.396994 |
Target: 5'- cCCCCAuccuCCCCaCCUCcuccCCCUCCUCu -3' miRNA: 3'- -GGGGUu---GGGG-GGAGauuuGGGGGGAG- -5' |
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27551 | 5' | -59.5 | NC_005881.1 | + | 184365 | 0.75 | 0.335017 |
Target: 5'- uCCCCAccaccucACCaCCCC-CUAAACCCCuCCUa -3' miRNA: 3'- -GGGGU-------UGG-GGGGaGAUUUGGGG-GGAg -5' |
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27551 | 5' | -59.5 | NC_005881.1 | + | 207412 | 0.75 | 0.335017 |
Target: 5'- uCCCCAccaccucACCaCCCC-CUAAACCCCuCCUa -3' miRNA: 3'- -GGGGU-------UGG-GGGGaGAUUUGGGG-GGAg -5' |
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27551 | 5' | -59.5 | NC_005881.1 | + | 184413 | 0.77 | 0.281676 |
Target: 5'- aCCCCGaugACCCCauggggucgaCCUUU-GACCCCCCUCu -3' miRNA: 3'- -GGGGU---UGGGG----------GGAGAuUUGGGGGGAG- -5' |
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27551 | 5' | -59.5 | NC_005881.1 | + | 207364 | 0.77 | 0.281676 |
Target: 5'- aCCCCGaugACCCCauggggucgaCCUUU-GACCCCCCUCu -3' miRNA: 3'- -GGGGU---UGGGG----------GGAGAuUUGGGGGGAG- -5' |
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27551 | 5' | -59.5 | NC_005881.1 | + | 207439 | 1.1 | 0.001727 |
Target: 5'- uCCCCAACCCCCCUCUAAACCCCCCUCu -3' miRNA: 3'- -GGGGUUGGGGGGAGAUUUGGGGGGAG- -5' |
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27551 | 5' | -59.5 | NC_005881.1 | + | 184338 | 1.1 | 0.001727 |
Target: 5'- uCCCCAACCCCCCUCUAAACCCCCCUCu -3' miRNA: 3'- -GGGGUUGGGGGGAGAUUUGGGGGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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