Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27552 | 5' | -53.4 | NC_005881.1 | + | 4562 | 0.66 | 0.981355 |
Target: 5'- -aAAUUCCUuggCCCCCAUGuGGUUuuuUUAa -3' miRNA: 3'- aaUUGAGGA---GGGGGUACuCCAGu--AGU- -5' |
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27552 | 5' | -53.4 | NC_005881.1 | + | 178475 | 0.66 | 0.981355 |
Target: 5'- -aAAUUCCUuggCCCCCAUGuGGUUuuuUUAa -3' miRNA: 3'- aaUUGAGGA---GGGGGUACuCCAGu--AGU- -5' |
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27552 | 5' | -53.4 | NC_005881.1 | + | 165510 | 0.67 | 0.968527 |
Target: 5'- ---uUUCCggCCCUA-GAGGUCAUCAu -3' miRNA: 3'- aauuGAGGagGGGGUaCUCCAGUAGU- -5' |
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27552 | 5' | -53.4 | NC_005881.1 | + | 197675 | 0.67 | 0.95449 |
Target: 5'- uUUAACUCCUCCCCCuuccgacuguaGUGAuuGGUUu--- -3' miRNA: 3'- -AAUUGAGGAGGGGG-----------UACU--CCAGuagu -5' |
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27552 | 5' | -53.4 | NC_005881.1 | + | 194101 | 0.67 | 0.95449 |
Target: 5'- uUUAACUCCUCCCCCuuccgacuguaGUGAuuGGUUu--- -3' miRNA: 3'- -AAUUGAGGAGGGGG-----------UACU--CCAGuagu -5' |
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27552 | 5' | -53.4 | NC_005881.1 | + | 184410 | 0.68 | 0.931684 |
Target: 5'- gUGACcCCgaugaCCCCAUGGGGUCGa-- -3' miRNA: 3'- aAUUGaGGag---GGGGUACUCCAGUagu -5' |
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27552 | 5' | -53.4 | NC_005881.1 | + | 207367 | 0.68 | 0.931684 |
Target: 5'- gUGACcCCgaugaCCCCAUGGGGUCGa-- -3' miRNA: 3'- aAUUGaGGag---GGGGUACUCCAGUagu -5' |
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27552 | 5' | -53.4 | NC_005881.1 | + | 158054 | 0.69 | 0.920857 |
Target: 5'- --cAC-CCUCUCCCAUGAGuGUCGaCAc -3' miRNA: 3'- aauUGaGGAGGGGGUACUC-CAGUaGU- -5' |
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27552 | 5' | -53.4 | NC_005881.1 | + | 207130 | 0.7 | 0.852424 |
Target: 5'- -aAGgUCga-CCCCAUGGGGUCAUCGg -3' miRNA: 3'- aaUUgAGgagGGGGUACUCCAGUAGU- -5' |
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27552 | 5' | -53.4 | NC_005881.1 | + | 184646 | 0.7 | 0.852424 |
Target: 5'- -aAGgUCga-CCCCAUGGGGUCAUCGg -3' miRNA: 3'- aaUUgAGgagGGGGUACUCCAGUAGU- -5' |
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27552 | 5' | -53.4 | NC_005881.1 | + | 189915 | 0.71 | 0.819022 |
Target: 5'- --uACUCCUCCUaCCAUGgAGGUCAa-- -3' miRNA: 3'- aauUGAGGAGGG-GGUAC-UCCAGUagu -5' |
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27552 | 5' | -53.4 | NC_005881.1 | + | 201861 | 0.71 | 0.819022 |
Target: 5'- --uACUCCUCCUaCCAUGgAGGUCAa-- -3' miRNA: 3'- aauUGAGGAGGG-GGUAC-UCCAGUagu -5' |
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27552 | 5' | -53.4 | NC_005881.1 | + | 193421 | 0.71 | 0.810215 |
Target: 5'- -aGGCUCCUCCUCCAUcucagguugcaGAGGU-GUCAu -3' miRNA: 3'- aaUUGAGGAGGGGGUA-----------CUCCAgUAGU- -5' |
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27552 | 5' | -53.4 | NC_005881.1 | + | 198356 | 0.71 | 0.810215 |
Target: 5'- -aGGCUCCUCCUCCAUcucagguugcaGAGGU-GUCAu -3' miRNA: 3'- aaUUGAGGAGGGGGUA-----------CUCCAgUAGU- -5' |
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27552 | 5' | -53.4 | NC_005881.1 | + | 184451 | 1.06 | 0.008499 |
Target: 5'- cUUAACUCCUCCCCCAUGAGGUCAUCAa -3' miRNA: 3'- -AAUUGAGGAGGGGGUACUCCAGUAGU- -5' |
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27552 | 5' | -53.4 | NC_005881.1 | + | 207326 | 1.06 | 0.008499 |
Target: 5'- cUUAACUCCUCCCCCAUGAGGUCAUCAa -3' miRNA: 3'- -AAUUGAGGAGGGGGUACUCCAGUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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