Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27554 | 3' | -43.7 | NC_005881.1 | + | 184601 | 0.67 | 1 |
Target: 5'- gCCGCAUUAa-----GUGG-UGGGAGGa -3' miRNA: 3'- -GGUGUAAUggaauaUACCuAUCCUCC- -5' |
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27554 | 3' | -43.7 | NC_005881.1 | + | 207176 | 0.67 | 1 |
Target: 5'- gCCGCAUUAa-----GUGG-UGGGAGGa -3' miRNA: 3'- -GGUGUAAUggaauaUACCuAUCCUCC- -5' |
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27554 | 3' | -43.7 | NC_005881.1 | + | 124879 | 0.66 | 1 |
Target: 5'- gCCACAguUUGCaCUg--GUGGu--GGAGGu -3' miRNA: 3'- -GGUGU--AAUG-GAauaUACCuauCCUCC- -5' |
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27554 | 3' | -43.7 | NC_005881.1 | + | 201627 | 0.69 | 0.999999 |
Target: 5'- aCCAUug-ACCUcc-AUGG-UAGGAGGa -3' miRNA: 3'- -GGUGuaaUGGAauaUACCuAUCCUCC- -5' |
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27554 | 3' | -43.7 | NC_005881.1 | + | 186725 | 0.69 | 0.999999 |
Target: 5'- cCCAUGUUAUUaUUAUucAUGGggGGGGGGg -3' miRNA: 3'- -GGUGUAAUGG-AAUA--UACCuaUCCUCC- -5' |
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27554 | 3' | -43.7 | NC_005881.1 | + | 190149 | 0.69 | 0.999999 |
Target: 5'- aCCAUug-ACCUcc-AUGG-UAGGAGGa -3' miRNA: 3'- -GGUGuaaUGGAauaUACCuAUCCUCC- -5' |
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27554 | 3' | -43.7 | NC_005881.1 | + | 205051 | 0.69 | 0.999999 |
Target: 5'- cCCAUGUUAUUaUUAUucAUGGggGGGGGGg -3' miRNA: 3'- -GGUGUAAUGG-AAUA--UACCuaUCCUCC- -5' |
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27554 | 3' | -43.7 | NC_005881.1 | + | 63996 | 0.7 | 0.999979 |
Target: 5'- uUCACGUUuCCUUuuugAUGGA--GGAGGa -3' miRNA: 3'- -GGUGUAAuGGAAua--UACCUauCCUCC- -5' |
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27554 | 3' | -43.7 | NC_005881.1 | + | 184481 | 0.74 | 0.998805 |
Target: 5'- aCCu--UUACCUUAUAUGGAaguaauGGGGGc -3' miRNA: 3'- -GGuguAAUGGAAUAUACCUau----CCUCC- -5' |
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27554 | 3' | -43.7 | NC_005881.1 | + | 207296 | 0.74 | 0.998805 |
Target: 5'- aCCu--UUACCUUAUAUGGAaguaauGGGGGc -3' miRNA: 3'- -GGuguAAUGGAAUAUACCUau----CCUCC- -5' |
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27554 | 3' | -43.7 | NC_005881.1 | + | 207025 | 1.16 | 0.031064 |
Target: 5'- aCCACAUUACCUUAUAUGGAUAGGAGGu -3' miRNA: 3'- -GGUGUAAUGGAAUAUACCUAUCCUCC- -5' |
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27554 | 3' | -43.7 | NC_005881.1 | + | 184751 | 1.16 | 0.031064 |
Target: 5'- aCCACAUUACCUUAUAUGGAUAGGAGGu -3' miRNA: 3'- -GGUGUAAUGGAAUAUACCUAUCCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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