Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27555 | 5' | -55.3 | NC_005882.1 | + | 17928 | 0.66 | 0.600607 |
Target: 5'- aGACCggcgaGCUGCGCGaggucgcgacGUUCCGCaCGUcGACc -3' miRNA: 3'- -UUGG-----CGAUGCGC----------CAAGGUG-GCAaCUG- -5' |
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27555 | 5' | -55.3 | NC_005882.1 | + | 18202 | 0.66 | 0.566635 |
Target: 5'- aGAUCGCgcagGCGCuGGUgcuggUCACCGagGACa -3' miRNA: 3'- -UUGGCGa---UGCG-CCAa----GGUGGCaaCUG- -5' |
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27555 | 5' | -55.3 | NC_005882.1 | + | 12063 | 0.66 | 0.555413 |
Target: 5'- gAGCCGCUggcGCGCGGa--CGCCGUcaGCg -3' miRNA: 3'- -UUGGCGA---UGCGCCaagGUGGCAacUG- -5' |
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27555 | 5' | -55.3 | NC_005882.1 | + | 12599 | 0.66 | 0.544258 |
Target: 5'- cGCCGCaGCGcCGGccgCCGCCGc-GACa -3' miRNA: 3'- uUGGCGaUGC-GCCaa-GGUGGCaaCUG- -5' |
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27555 | 5' | -55.3 | NC_005882.1 | + | 22199 | 0.66 | 0.544258 |
Target: 5'- gAGCCGa-ACGaCGGa-CCGCUGUUGACg -3' miRNA: 3'- -UUGGCgaUGC-GCCaaGGUGGCAACUG- -5' |
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27555 | 5' | -55.3 | NC_005882.1 | + | 27628 | 0.66 | 0.544258 |
Target: 5'- cGGCCGCgcGCGCGGcgaCCGCCGacGAg -3' miRNA: 3'- -UUGGCGa-UGCGCCaa-GGUGGCaaCUg -5' |
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27555 | 5' | -55.3 | NC_005882.1 | + | 27222 | 0.67 | 0.529867 |
Target: 5'- cACCGCgauCGCGGUcggcgcguaccucaUCUACCGcuacugGACg -3' miRNA: 3'- uUGGCGau-GCGCCA--------------AGGUGGCaa----CUG- -5' |
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27555 | 5' | -55.3 | NC_005882.1 | + | 28913 | 0.67 | 0.522175 |
Target: 5'- uGCCGCU-CGCGaGaaCCGCCGUaaucGGCa -3' miRNA: 3'- uUGGCGAuGCGC-CaaGGUGGCAa---CUG- -5' |
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27555 | 5' | -55.3 | NC_005882.1 | + | 4676 | 0.69 | 0.415131 |
Target: 5'- uGCCGCUGCgcgaacgagcugcaGCGGUU--GCUGUUGACc -3' miRNA: 3'- uUGGCGAUG--------------CGCCAAggUGGCAACUG- -5' |
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27555 | 5' | -55.3 | NC_005882.1 | + | 1357 | 0.7 | 0.361966 |
Target: 5'- uGCgCGCUuccuCGCGG--CCACCGUUGAg -3' miRNA: 3'- uUG-GCGAu---GCGCCaaGGUGGCAACUg -5' |
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27555 | 5' | -55.3 | NC_005882.1 | + | 28369 | 0.7 | 0.344467 |
Target: 5'- gAAUCGUgccucGCGCGGUggCCACCGccgaugUGACg -3' miRNA: 3'- -UUGGCGa----UGCGCCAa-GGUGGCa-----ACUG- -5' |
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27555 | 5' | -55.3 | NC_005882.1 | + | 27576 | 0.71 | 0.295682 |
Target: 5'- cGCCGCgcGCGCGG--CCGCCGgcaUGGCg -3' miRNA: 3'- uUGGCGa-UGCGCCaaGGUGGCa--ACUG- -5' |
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27555 | 5' | -55.3 | NC_005882.1 | + | 30158 | 0.72 | 0.25927 |
Target: 5'- cGCCGCUacggGCGUGGUUCCAacaaCGccGACg -3' miRNA: 3'- uUGGCGA----UGCGCCAAGGUg---GCaaCUG- -5' |
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27555 | 5' | -55.3 | NC_005882.1 | + | 15742 | 0.76 | 0.140646 |
Target: 5'- cAACCGCUACgGCGGcgUCGCCGU-GGCg -3' miRNA: 3'- -UUGGCGAUG-CGCCaaGGUGGCAaCUG- -5' |
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27555 | 5' | -55.3 | NC_005882.1 | + | 28327 | 0.77 | 0.121709 |
Target: 5'- aGACCGcCUGCGCGG--CCGCCGgcGGCa -3' miRNA: 3'- -UUGGC-GAUGCGCCaaGGUGGCaaCUG- -5' |
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27555 | 5' | -55.3 | NC_005882.1 | + | 10082 | 0.77 | 0.121709 |
Target: 5'- uGAUCGCUACGCGGUUCaGCCag-GACg -3' miRNA: 3'- -UUGGCGAUGCGCCAAGgUGGcaaCUG- -5' |
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27555 | 5' | -55.3 | NC_005882.1 | + | 16981 | 0.85 | 0.029708 |
Target: 5'- cGGCCGCUGC-CGGUgUCCGCCGUUGAUa -3' miRNA: 3'- -UUGGCGAUGcGCCA-AGGUGGCAACUG- -5' |
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27555 | 5' | -55.3 | NC_005882.1 | + | 249 | 1.06 | 0.000848 |
Target: 5'- cAACCGCUACGCGGUUCCACCGUUGACc -3' miRNA: 3'- -UUGGCGAUGCGCCAAGGUGGCAACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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