Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27556 | 5' | -55 | NC_005882.1 | + | 9887 | 0.65 | 0.591901 |
Target: 5'- -gCUGgUCgGGCAgaUCGCCGACGCGa-- -3' miRNA: 3'- aaGGCgAG-CUGU--AGCGGUUGCGCauu -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 6968 | 0.66 | 0.557647 |
Target: 5'- ---aGCUCGACGauaUCGCCAccaugaGCGUGAa -3' miRNA: 3'- aaggCGAGCUGU---AGCGGUug----CGCAUU- -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 17177 | 0.66 | 0.535124 |
Target: 5'- aUCCGCUuuaaaagcggcgCGACcagCGCCuGCGCGUc- -3' miRNA: 3'- aAGGCGA------------GCUGua-GCGGuUGCGCAuu -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 8002 | 0.67 | 0.523981 |
Target: 5'- --gCGCUCGACGUUGCCGcgAUGCc--- -3' miRNA: 3'- aagGCGAGCUGUAGCGGU--UGCGcauu -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 1911 | 0.67 | 0.523981 |
Target: 5'- --gUGCUCGAUGUCGCgGuuACGCGUu- -3' miRNA: 3'- aagGCGAGCUGUAGCGgU--UGCGCAuu -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 5211 | 0.67 | 0.501972 |
Target: 5'- -cCUGggCGGCAUCGUCAcgccacggauuGCGCGUGAu -3' miRNA: 3'- aaGGCgaGCUGUAGCGGU-----------UGCGCAUU- -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 28365 | 0.67 | 0.501972 |
Target: 5'- gUCCGCgaugcgCGugGcCGUCGACGCGg-- -3' miRNA: 3'- aAGGCGa-----GCugUaGCGGUUGCGCauu -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 19662 | 0.67 | 0.49112 |
Target: 5'- -gCUGCUCGGCAuacgacacaugcUCGCUGugGCGg-- -3' miRNA: 3'- aaGGCGAGCUGU------------AGCGGUugCGCauu -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 10705 | 0.68 | 0.448869 |
Target: 5'- -gCCGCUCGAUccgguaucacaAcCGCCGGCGCGc-- -3' miRNA: 3'- aaGGCGAGCUG-----------UaGCGGUUGCGCauu -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 30133 | 0.68 | 0.438621 |
Target: 5'- --aCGC-CGACGUCGCCuuuCGCGg-- -3' miRNA: 3'- aagGCGaGCUGUAGCGGuu-GCGCauu -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 17082 | 0.68 | 0.438621 |
Target: 5'- gUCCGCgcgCGACcugaaUGCCGACGCGc-- -3' miRNA: 3'- aAGGCGa--GCUGua---GCGGUUGCGCauu -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 23566 | 0.69 | 0.38948 |
Target: 5'- -aCCGCacggCGGCAUgGCCGAC-CGUGAc -3' miRNA: 3'- aaGGCGa---GCUGUAgCGGUUGcGCAUU- -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 21164 | 0.69 | 0.38948 |
Target: 5'- -cCUGCUCGGCGUCauccuggucaugGCCGGCGCa--- -3' miRNA: 3'- aaGGCGAGCUGUAG------------CGGUUGCGcauu -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 10509 | 0.69 | 0.370859 |
Target: 5'- aUUCCGaCUCcaccaacaagGGCGU-GCCGACGCGUGAc -3' miRNA: 3'- -AAGGC-GAG----------CUGUAgCGGUUGCGCAUU- -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 33054 | 0.7 | 0.343218 |
Target: 5'- -aCUGCUCGACcugCGCCuucagguAGCGCGUGc -3' miRNA: 3'- aaGGCGAGCUGua-GCGG-------UUGCGCAUu -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 19770 | 0.7 | 0.318733 |
Target: 5'- -gCUGCUUGGCAUCGCCuuCGCc--- -3' miRNA: 3'- aaGGCGAGCUGUAGCGGuuGCGcauu -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 21739 | 0.71 | 0.287151 |
Target: 5'- gUCCGCgcgCGcCGUCGCCAguucgACGCGg-- -3' miRNA: 3'- aAGGCGa--GCuGUAGCGGU-----UGCGCauu -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 29400 | 0.73 | 0.234022 |
Target: 5'- gUUCCGCUucgccacgcgagccgCGAuCGUCGCCAGCGUGg-- -3' miRNA: 3'- -AAGGCGA---------------GCU-GUAGCGGUUGCGCauu -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 18988 | 0.74 | 0.185169 |
Target: 5'- aUCCGCgCGACAacgCGCCGGCGUGg-- -3' miRNA: 3'- aAGGCGaGCUGUa--GCGGUUGCGCauu -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 15740 | 0.75 | 0.160553 |
Target: 5'- -aCCGCUacggCGGCGUCGCCGugGCGc-- -3' miRNA: 3'- aaGGCGA----GCUGUAGCGGUugCGCauu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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