Results 21 - 22 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27556 | 5' | -55 | NC_005882.1 | + | 12421 | 0.78 | 0.106619 |
Target: 5'- -aCCGCUCGACAUgGCCGAaccaGCGUu- -3' miRNA: 3'- aaGGCGAGCUGUAgCGGUUg---CGCAuu -5' |
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27556 | 5' | -55 | NC_005882.1 | + | 1988 | 1.04 | 0.001262 |
Target: 5'- aUUCCGCUCGACAUCGCCAACGCGUAAc -3' miRNA: 3'- -AAGGCGAGCUGUAGCGGUUGCGCAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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