Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27557 | 5' | -57.4 | NC_005882.1 | + | 25992 | 0.66 | 0.45546 |
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Target: 5'- aGGAGGCGuucaaccGCGcGGCCuaCGGCGGc -3' miRNA: 3'- aCCUCCGUua-----CGC-UCGGuaGCCGCUu -5' |
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| 27557 | 5' | -57.4 | NC_005882.1 | + | 7190 | 0.67 | 0.377779 |
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Target: 5'- aGGAGGC-AUGUGAGCa--CGGCc-- -3' miRNA: 3'- aCCUCCGuUACGCUCGguaGCCGcuu -5' |
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| 27557 | 5' | -57.4 | NC_005882.1 | + | 6029 | 0.67 | 0.368713 |
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Target: 5'- cUGGGGcCGGUGCGAGCaGUUGGuCGGGa -3' miRNA: 3'- -ACCUCcGUUACGCUCGgUAGCC-GCUU- -5' |
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| 27557 | 5' | -57.4 | NC_005882.1 | + | 19712 | 0.67 | 0.368713 |
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Target: 5'- cGGcgaAGGCGAUGCcaagcAGCCGcUGGCGAGc -3' miRNA: 3'- aCC---UCCGUUACGc----UCGGUaGCCGCUU- -5' |
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| 27557 | 5' | -57.4 | NC_005882.1 | + | 21082 | 0.67 | 0.368713 |
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Target: 5'- aUGGuGGCGuGUGCGcAGCCGgcgcgaaggcgcUCGGCGu- -3' miRNA: 3'- -ACCuCCGU-UACGC-UCGGU------------AGCCGCuu -5' |
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| 27557 | 5' | -57.4 | NC_005882.1 | + | 23157 | 0.67 | 0.359798 |
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Target: 5'- cUGGAGGCGAU---GGCCGUCcagcucggGGCGAu -3' miRNA: 3'- -ACCUCCGUUAcgcUCGGUAG--------CCGCUu -5' |
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| 27557 | 5' | -57.4 | NC_005882.1 | + | 8867 | 0.74 | 0.128477 |
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Target: 5'- -cGAGGCAAUGCGAGCgCuuuugccggGUCGGCGc- -3' miRNA: 3'- acCUCCGUUACGCUCG-G---------UAGCCGCuu -5' |
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| 27557 | 5' | -57.4 | NC_005882.1 | + | 3680 | 0.74 | 0.12055 |
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Target: 5'- cGGAGGUAuucguccaggcGCGGGCCGUCGaGCGGGg -3' miRNA: 3'- aCCUCCGUua---------CGCUCGGUAGC-CGCUU- -5' |
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| 27557 | 5' | -57.4 | NC_005882.1 | + | 2090 | 1.06 | 0.000461 |
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Target: 5'- cUGGAGGCAAUGCGAGCCAUCGGCGAAg -3' miRNA: 3'- -ACCUCCGUUACGCUCGGUAGCCGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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