Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27559 | 5' | -63.7 | NC_005882.1 | + | 33727 | 0.66 | 0.234516 |
Target: 5'- cGCUGCacgaauccggucgucGCGAGUuucgucGUGUugucgaauuGcGGCGGCGUCa -3' miRNA: 3'- aCGACG---------------CGCUCG------CACG---------C-CCGCCGCAG- -5' |
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27559 | 5' | -63.7 | NC_005882.1 | + | 8653 | 0.66 | 0.232122 |
Target: 5'- gGCUGCGuCGAcgGCGUacgcuuuucGCaGGCGcuGCGUCg -3' miRNA: 3'- aCGACGC-GCU--CGCA---------CGcCCGC--CGCAG- -5' |
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27559 | 5' | -63.7 | NC_005882.1 | + | 11996 | 0.66 | 0.232122 |
Target: 5'- gGCacuUGCGCG-GCcacggGCGcGGCGGCGcCg -3' miRNA: 3'- aCG---ACGCGCuCGca---CGC-CCGCCGCaG- -5' |
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27559 | 5' | -63.7 | NC_005882.1 | + | 15260 | 0.66 | 0.232122 |
Target: 5'- gGgaGCGCcguGAGCa-GCGGGCcGaGCGUCg -3' miRNA: 3'- aCgaCGCG---CUCGcaCGCCCG-C-CGCAG- -5' |
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27559 | 5' | -63.7 | NC_005882.1 | + | 15984 | 0.66 | 0.220459 |
Target: 5'- aGCUGCGCGcggacGCGcacgucgGCGGGU-GCGUg -3' miRNA: 3'- aCGACGCGCu----CGCa------CGCCCGcCGCAg -5' |
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27559 | 5' | -63.7 | NC_005882.1 | + | 17151 | 0.66 | 0.204437 |
Target: 5'- cGcCUGCGCGucggcauucaggucGCGcGCGGacaagguguucaguGCGGCGUCg -3' miRNA: 3'- aC-GACGCGCu-------------CGCaCGCC--------------CGCCGCAG- -5' |
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27559 | 5' | -63.7 | NC_005882.1 | + | 1796 | 0.66 | 0.203903 |
Target: 5'- cGCUGCGCaaGGGCGacacggugacgcUGaCGGGauuCGGCGUa -3' miRNA: 3'- aCGACGCG--CUCGC------------AC-GCCC---GCCGCAg -5' |
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27559 | 5' | -63.7 | NC_005882.1 | + | 15499 | 0.67 | 0.195522 |
Target: 5'- aUGCUGUccgagucgugguaucGCGAGCacaugccgcccGUGaaGGCGGCGUUc -3' miRNA: 3'- -ACGACG---------------CGCUCG-----------CACgcCCGCCGCAG- -5' |
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27559 | 5' | -63.7 | NC_005882.1 | + | 10130 | 0.67 | 0.193474 |
Target: 5'- cGUUGUcccgaGCGGGCGUagauUGGGCGGCGc- -3' miRNA: 3'- aCGACG-----CGCUCGCAc---GCCCGCCGCag -5' |
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27559 | 5' | -63.7 | NC_005882.1 | + | 3381 | 0.67 | 0.193474 |
Target: 5'- uUGC-GCGCGGGCGcUGCGcGGCcugGGCu-- -3' miRNA: 3'- -ACGaCGCGCUCGC-ACGC-CCG---CCGcag -5' |
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27559 | 5' | -63.7 | NC_005882.1 | + | 3956 | 0.67 | 0.188437 |
Target: 5'- aGCUGCGCGaAGCGggcuucCGGGCuGC-UCu -3' miRNA: 3'- aCGACGCGC-UCGCac----GCCCGcCGcAG- -5' |
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27559 | 5' | -63.7 | NC_005882.1 | + | 1620 | 0.67 | 0.183515 |
Target: 5'- aGCUGCGCG-GCGacgcccgaUGCGaugGGCGUCg -3' miRNA: 3'- aCGACGCGCuCGC--------ACGCccgCCGCAG- -5' |
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27559 | 5' | -63.7 | NC_005882.1 | + | 27312 | 0.68 | 0.16495 |
Target: 5'- cGCguuCGCGGGUGUGCucGGGUgguucGGCGUUg -3' miRNA: 3'- aCGac-GCGCUCGCACG--CCCG-----CCGCAG- -5' |
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27559 | 5' | -63.7 | NC_005882.1 | + | 25594 | 0.68 | 0.152151 |
Target: 5'- cGCUGCGUGAGCaacGCcagGGGCucGGCGcCg -3' miRNA: 3'- aCGACGCGCUCGca-CG---CCCG--CCGCaG- -5' |
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27559 | 5' | -63.7 | NC_005882.1 | + | 7336 | 0.68 | 0.142956 |
Target: 5'- cGCUGCGuCGAccauUGCGGGCaugcccggaacaucGGCGUCg -3' miRNA: 3'- aCGACGC-GCUcgc-ACGCCCG--------------CCGCAG- -5' |
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27559 | 5' | -63.7 | NC_005882.1 | + | 12516 | 0.7 | 0.115761 |
Target: 5'- cUGCUuucGCGCGgccAGUGUGuCGcGGCGGCGg- -3' miRNA: 3'- -ACGA---CGCGC---UCGCAC-GC-CCGCCGCag -5' |
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27559 | 5' | -63.7 | NC_005882.1 | + | 2083 | 0.7 | 0.100771 |
Target: 5'- cGCaGCGCGAGCacGCGGcCGGUGUUg -3' miRNA: 3'- aCGaCGCGCUCGcaCGCCcGCCGCAG- -5' |
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27559 | 5' | -63.7 | NC_005882.1 | + | 7690 | 0.79 | 0.022782 |
Target: 5'- aUGCUGU-CGAgGCGcucGCGGGCGGCGUCg -3' miRNA: 3'- -ACGACGcGCU-CGCa--CGCCCGCCGCAG- -5' |
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27559 | 5' | -63.7 | NC_005882.1 | + | 3266 | 1.09 | 7.9e-05 |
Target: 5'- gUGCUGCGCGAGCGUGCGGGCGGCGUCa -3' miRNA: 3'- -ACGACGCGCUCGCACGCCCGCCGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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