Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2756 | 3' | -54.3 | NC_001491.2 | + | 143598 | 0.7 | 0.817022 |
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Target: 5'- uCCUCccCGGGAAGcGCGuCGCGCCAacGGg -3' miRNA: 3'- cGGAGaaGUCCUUU-CGC-GCGUGGU--CC- -5' |
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| 2756 | 3' | -54.3 | NC_001491.2 | + | 116696 | 0.7 | 0.808203 |
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Target: 5'- gGCCgCUcCGGGGAccAGgGCGC-CCAGGc -3' miRNA: 3'- -CGGaGAaGUCCUU--UCgCGCGuGGUCC- -5' |
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| 2756 | 3' | -54.3 | NC_001491.2 | + | 103637 | 0.7 | 0.808203 |
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Target: 5'- aCCUcCUUguGGGAcgaguacaGGCGCGUccaGCCGGGa -3' miRNA: 3'- cGGA-GAAguCCUU--------UCGCGCG---UGGUCC- -5' |
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| 2756 | 3' | -54.3 | NC_001491.2 | + | 138525 | 0.7 | 0.79922 |
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Target: 5'- uGCUUCUUcCGGGAGAGUgcugggguccGCGCugCuGGc -3' miRNA: 3'- -CGGAGAA-GUCCUUUCG----------CGCGugGuCC- -5' |
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| 2756 | 3' | -54.3 | NC_001491.2 | + | 53219 | 0.7 | 0.79922 |
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Target: 5'- aGCC-CggcgUCGGGAGGGCcguGgGCuACCAGGg -3' miRNA: 3'- -CGGaGa---AGUCCUUUCG---CgCG-UGGUCC- -5' |
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| 2756 | 3' | -54.3 | NC_001491.2 | + | 146488 | 0.71 | 0.742405 |
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Target: 5'- aGCCUUgcgUCGGGGcccgaGGGcCGCGCAgacCCGGGu -3' miRNA: 3'- -CGGAGa--AGUCCU-----UUC-GCGCGU---GGUCC- -5' |
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| 2756 | 3' | -54.3 | NC_001491.2 | + | 146332 | 0.72 | 0.721578 |
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Target: 5'- cGCCUCgucucUCGGGuAGGCcauguccgcguagGCGCGCCGGa -3' miRNA: 3'- -CGGAGa----AGUCCuUUCG-------------CGCGUGGUCc -5' |
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| 2756 | 3' | -54.3 | NC_001491.2 | + | 143714 | 0.73 | 0.640845 |
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Target: 5'- uCCUCc-CAGGGAAGCGCGUcgcGCCAacGGg -3' miRNA: 3'- cGGAGaaGUCCUUUCGCGCG---UGGU--CC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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