Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2756 | 3' | -54.3 | NC_001491.2 | + | 60581 | 0.66 | 0.944824 |
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Target: 5'- aGCCUCUgCAGGAGcGGCGCaGaCAUguGa -3' miRNA: 3'- -CGGAGAaGUCCUU-UCGCG-C-GUGguCc -5' |
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| 2756 | 3' | -54.3 | NC_001491.2 | + | 25759 | 0.66 | 0.939736 |
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Target: 5'- cGCUUCcggUCAucgacaacccaguGGAGAGacaGCGCGCCAuGGc -3' miRNA: 3'- -CGGAGa--AGU-------------CCUUUCg--CGCGUGGU-CC- -5' |
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| 2756 | 3' | -54.3 | NC_001491.2 | + | 116663 | 0.66 | 0.939736 |
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Target: 5'- cGCCUUcagacUCAGGGAGcucggggacgccuGCGUGCuggccuGCCAGGc -3' miRNA: 3'- -CGGAGa----AGUCCUUU-------------CGCGCG------UGGUCC- -5' |
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| 2756 | 3' | -54.3 | NC_001491.2 | + | 147705 | 0.67 | 0.934355 |
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Target: 5'- gGCCUggggaucgaAGGuGAGCGCcggGCGCCAGGc -3' miRNA: 3'- -CGGAgaag-----UCCuUUCGCG---CGUGGUCC- -5' |
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| 2756 | 3' | -54.3 | NC_001491.2 | + | 72861 | 0.67 | 0.930256 |
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Target: 5'- gGCgUCUUUguGGGcgGGCGCGCcugGCuCGGGu -3' miRNA: 3'- -CGgAGAAG--UCCuuUCGCGCG---UG-GUCC- -5' |
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| 2756 | 3' | -54.3 | NC_001491.2 | + | 145415 | 0.67 | 0.913497 |
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Target: 5'- cGCCgaagCUggagCcGGAgcGgGUGCGCCGGGg -3' miRNA: 3'- -CGGa---GAa---GuCCUuuCgCGCGUGGUCC- -5' |
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| 2756 | 3' | -54.3 | NC_001491.2 | + | 148178 | 0.68 | 0.894563 |
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Target: 5'- uCCUCggu-GGAGAccGCGUccaGCACCAGGa -3' miRNA: 3'- cGGAGaaguCCUUU--CGCG---CGUGGUCC- -5' |
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| 2756 | 3' | -54.3 | NC_001491.2 | + | 143714 | 0.73 | 0.640845 |
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Target: 5'- uCCUCc-CAGGGAAGCGCGUcgcGCCAacGGg -3' miRNA: 3'- cGGAGaaGUCCUUUCGCGCG---UGGU--CC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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