Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27560 | 5' | -54 | NC_005882.1 | + | 21102 | 0.66 | 0.721104 |
Target: 5'- gGCGCGAaggcgcUCGgcguUGCCGAAACGaccgccgaUGUCGg -3' miRNA: 3'- -UGCGCU------AGC----ACGGCUUUGCc-------ACAGCg -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 23400 | 0.66 | 0.714527 |
Target: 5'- cACGCGAUgagagcggcuccaggCGUuCCGcguuggucGCGGUGUCGUa -3' miRNA: 3'- -UGCGCUA---------------GCAcGGCuu------UGCCACAGCG- -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 19804 | 0.66 | 0.710127 |
Target: 5'- cGCGCGAgCGcGCCGGcgugacgacGACGGg--CGCc -3' miRNA: 3'- -UGCGCUaGCaCGGCU---------UUGCCacaGCG- -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 18023 | 0.66 | 0.686827 |
Target: 5'- uCGCGGcgCGUGaCUGGgucGACGGUgcggaacGUCGCg -3' miRNA: 3'- uGCGCUa-GCAC-GGCU---UUGCCA-------CAGCG- -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 26741 | 0.67 | 0.665535 |
Target: 5'- gGCGUGGUCGgcuucgcaacgGCCGugauCGGcaugaagGUCGCg -3' miRNA: 3'- -UGCGCUAGCa----------CGGCuuu-GCCa------CAGCG- -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 32112 | 0.67 | 0.665535 |
Target: 5'- aAC-CGAUCGUGCCGc--CGGccUGgggCGCg -3' miRNA: 3'- -UGcGCUAGCACGGCuuuGCC--ACa--GCG- -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 18535 | 0.67 | 0.665534 |
Target: 5'- cCGCGAcCGgcuugcccGCCGAAcUGGUGaaUCGCg -3' miRNA: 3'- uGCGCUaGCa-------CGGCUUuGCCAC--AGCG- -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 13140 | 0.67 | 0.653148 |
Target: 5'- gACGUucaucggccuucuGAUCGggGCCGGcaaGGUGUUGCu -3' miRNA: 3'- -UGCG-------------CUAGCa-CGGCUuugCCACAGCG- -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 21365 | 0.67 | 0.642994 |
Target: 5'- -aGCuGGcCGUGCCGGAACGGa--CGCu -3' miRNA: 3'- ugCG-CUaGCACGGCUUUGCCacaGCG- -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 26821 | 0.67 | 0.642994 |
Target: 5'- uCGCGAccuUCaUGCCGAucACGGccGUUGCg -3' miRNA: 3'- uGCGCU---AGcACGGCUu-UGCCa-CAGCG- -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 34568 | 0.67 | 0.631702 |
Target: 5'- gAUGaGAUCGuUGUCGAGacggcgcucgGCGGUGcCGCg -3' miRNA: 3'- -UGCgCUAGC-ACGGCUU----------UGCCACaGCG- -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 19913 | 0.67 | 0.609131 |
Target: 5'- cGCGCuucgGAUCGacguucucGCCGuacucGACGGUGUCGg -3' miRNA: 3'- -UGCG----CUAGCa-------CGGCu----UUGCCACAGCg -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 21999 | 0.68 | 0.597872 |
Target: 5'- uCGCGAUCGUGCUGcgccagcucAACGGc--CGCg -3' miRNA: 3'- uGCGCUAGCACGGCu--------UUGCCacaGCG- -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 29380 | 0.68 | 0.586643 |
Target: 5'- cCGCGAUCGUcGCCaGcgUGGUGU-GCu -3' miRNA: 3'- uGCGCUAGCA-CGGcUuuGCCACAgCG- -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 22702 | 0.68 | 0.575454 |
Target: 5'- gACGCGGUCuacgacuacgGCagCGGGACGGUGaUCGUg -3' miRNA: 3'- -UGCGCUAGca--------CG--GCUUUGCCAC-AGCG- -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 18991 | 0.68 | 0.564315 |
Target: 5'- cGCGCGAcaaCGcGCCGGcgUGGggcuggGUCGCu -3' miRNA: 3'- -UGCGCUa--GCaCGGCUuuGCCa-----CAGCG- -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 5777 | 0.68 | 0.564314 |
Target: 5'- aGCGCGG-CGggGCCGAccugcugcACGGUGUgccaCGCa -3' miRNA: 3'- -UGCGCUaGCa-CGGCUu-------UGCCACA----GCG- -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 34296 | 0.68 | 0.553233 |
Target: 5'- uAUGCGGUCGaGCCGAuguucgagaAACGGa-UCGCc -3' miRNA: 3'- -UGCGCUAGCaCGGCU---------UUGCCacAGCG- -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 20506 | 0.69 | 0.542217 |
Target: 5'- gACGCcgaccgGAUCGcUGCCGGccGCGGUgaacuucucgGUCGCg -3' miRNA: 3'- -UGCG------CUAGC-ACGGCUu-UGCCA----------CAGCG- -5' |
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27560 | 5' | -54 | NC_005882.1 | + | 15414 | 0.7 | 0.437194 |
Target: 5'- -gGCGAUCugcuccugcUGCCGGAACGGcacGUUGCg -3' miRNA: 3'- ugCGCUAGc--------ACGGCUUUGCCa--CAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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