miRNA: 3'- -GCGACU--------------A---CGCGCUGGAGCuUCUGGCg -5'
miRNA: 3'- gCGAC------UACgCGCUG---GAGCU--------UCUGGCG- -5'
miRNA: 3'- gCGAcuaCGCGCUggAGCuuCUGGCG- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27561 | 3' | -55.8 | NC_005882.1 | + | 19468 | 0.66 | 0.604541 |
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Target: 5'- gCGCUGGucggcgcaucgucuUgucGCGCGACUUCGcGGAUCGa -3' miRNA: 3'- -GCGACU--------------A---CGCGCUGGAGCuUCUGGCg -5' |
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| 27561 | 3' | -55.8 | NC_005882.1 | + | 27962 | 0.66 | 0.607895 |
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Target: 5'- gGCUGcaacgcAUGgGCGACaagCUCGAcgagauucGGAUCGCg -3' miRNA: 3'- gCGAC------UACgCGCUG---GAGCU--------UCUGGCG- -5' |
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| 27561 | 3' | -55.8 | NC_005882.1 | + | 660 | 0.66 | 0.607895 |
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Target: 5'- aGCUcggGCGCGAgaUUGccGACCGCa -3' miRNA: 3'- gCGAcuaCGCGCUggAGCuuCUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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