Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27563 | 5' | -56.5 | NC_005882.1 | + | 8651 | 0.66 | 0.522479 |
Target: 5'- aGGGCuGcGUCGACGGCGuacgcuuuucgcaGgCGCUGCGu -3' miRNA: 3'- gCUCGcC-CAGUUGUCGU-------------UgGCGACGU- -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 7707 | 0.66 | 0.512735 |
Target: 5'- gCGGGCGGcGUCGaggcuacggaggAUGGCGACgGCgugGCGc -3' miRNA: 3'- -GCUCGCC-CAGU------------UGUCGUUGgCGa--CGU- -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 17038 | 0.66 | 0.491361 |
Target: 5'- -cGGCGGacacCGGCAGCGGCCGCUcGUc -3' miRNA: 3'- gcUCGCCca--GUUGUCGUUGGCGA-CGu -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 6765 | 0.66 | 0.490303 |
Target: 5'- uCGAgGCGGaGUCGguuauucGCGGCAACCuGCgUGCc -3' miRNA: 3'- -GCU-CGCC-CAGU-------UGUCGUUGG-CG-ACGu -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 27853 | 0.66 | 0.48083 |
Target: 5'- gCGAGCaGGUCGucgAGCAugCGCUcCAg -3' miRNA: 3'- -GCUCGcCCAGUug-UCGUugGCGAcGU- -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 16466 | 0.67 | 0.470409 |
Target: 5'- cCGAuGCuGGUCGGCaAGCAuCCGC-GCAg -3' miRNA: 3'- -GCU-CGcCCAGUUG-UCGUuGGCGaCGU- -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 22089 | 0.67 | 0.460105 |
Target: 5'- uCGAGCacGUCGAUGGCAccGCCGCgGCc -3' miRNA: 3'- -GCUCGccCAGUUGUCGU--UGGCGaCGu -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 25708 | 0.67 | 0.449921 |
Target: 5'- aCGGGCGcGGcCGugGCAGCcGCCGUcGCGu -3' miRNA: 3'- -GCUCGC-CCaGU--UGUCGuUGGCGaCGU- -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 29707 | 0.67 | 0.430921 |
Target: 5'- gCGGGCaagcggaaacaguccGGUCAgucgacgaGCGGCGACaCGCUGCGg -3' miRNA: 3'- -GCUCGc--------------CCAGU--------UGUCGUUG-GCGACGU- -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 22463 | 0.67 | 0.420138 |
Target: 5'- gCGGGCuuGGUCGACGGC-GCUGCgGCc -3' miRNA: 3'- -GCUCGc-CCAGUUGUCGuUGGCGaCGu -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 15277 | 0.67 | 0.420138 |
Target: 5'- aCGAGCGGGcgUCGACcgggAGCG-CCGUgaGCAg -3' miRNA: 3'- -GCUCGCCC--AGUUG----UCGUuGGCGa-CGU- -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 177 | 0.68 | 0.410479 |
Target: 5'- gGAGacaGuGGUCAACGGUggAACCGCguaGCGg -3' miRNA: 3'- gCUCg--C-CCAGUUGUCG--UUGGCGa--CGU- -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 27557 | 0.68 | 0.410479 |
Target: 5'- uCGGGCGcucGUCGGCGGUcGCCGCgcgcGCGg -3' miRNA: 3'- -GCUCGCc--CAGUUGUCGuUGGCGa---CGU- -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 3707 | 0.69 | 0.346884 |
Target: 5'- uCGAGCGGGgccagcUCGACcauuucGC-AUCGCUGCAc -3' miRNA: 3'- -GCUCGCCC------AGUUGu-----CGuUGGCGACGU- -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 12237 | 0.7 | 0.30596 |
Target: 5'- gGAGCGcaUC-ACGGCGGCCGgUGCAu -3' miRNA: 3'- gCUCGCccAGuUGUCGUUGGCgACGU- -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 12377 | 0.7 | 0.283234 |
Target: 5'- uCGAGCGGuUCAACGccggccggcacGCGGCCGCUuuccGCGa -3' miRNA: 3'- -GCUCGCCcAGUUGU-----------CGUUGGCGA----CGU- -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 31371 | 0.7 | 0.283234 |
Target: 5'- gCGAGCuGGUCGGCGugacGCGGCUGCcgGCGc -3' miRNA: 3'- -GCUCGcCCAGUUGU----CGUUGGCGa-CGU- -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 30840 | 0.7 | 0.283234 |
Target: 5'- aGAGCuGGGUCAuGCGGCcauguuGCCGC-GCGa -3' miRNA: 3'- gCUCG-CCCAGU-UGUCGu-----UGGCGaCGU- -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 35230 | 0.7 | 0.283234 |
Target: 5'- aCGAGaaGGUCAucguucCAGCGcuuGCCGUUGCAa -3' miRNA: 3'- -GCUCgcCCAGUu-----GUCGU---UGGCGACGU- -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 27163 | 0.71 | 0.241826 |
Target: 5'- gGAcGCGGGcCAGCAGCAucCCGCccuggGCGa -3' miRNA: 3'- gCU-CGCCCaGUUGUCGUu-GGCGa----CGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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