Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27563 | 5' | -56.5 | NC_005882.1 | + | 6765 | 0.66 | 0.490303 |
Target: 5'- uCGAgGCGGaGUCGguuauucGCGGCAACCuGCgUGCc -3' miRNA: 3'- -GCU-CGCC-CAGU-------UGUCGUUGG-CG-ACGu -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 17038 | 0.66 | 0.491361 |
Target: 5'- -cGGCGGacacCGGCAGCGGCCGCUcGUc -3' miRNA: 3'- gcUCGCCca--GUUGUCGUUGGCGA-CGu -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 7707 | 0.66 | 0.512735 |
Target: 5'- gCGGGCGGcGUCGaggcuacggaggAUGGCGACgGCgugGCGc -3' miRNA: 3'- -GCUCGCC-CAGU------------UGUCGUUGgCGa--CGU- -5' |
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27563 | 5' | -56.5 | NC_005882.1 | + | 8651 | 0.66 | 0.522479 |
Target: 5'- aGGGCuGcGUCGACGGCGuacgcuuuucgcaGgCGCUGCGu -3' miRNA: 3'- gCUCGcC-CAGUUGUCGU-------------UgGCGACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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