Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27565 | 3' | -53.1 | NC_005882.1 | + | 26083 | 0.66 | 0.727421 |
Target: 5'- --cUUCaGCUcgaaccgGCCGAGcUUGCCGCc -3' miRNA: 3'- cuaAAGcCGAua-----CGGCUCuGACGGCG- -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 20657 | 0.66 | 0.704954 |
Target: 5'- ---gUCGGCgaagccaucUAUGCCGAcGACaagGaCCGCu -3' miRNA: 3'- cuaaAGCCG---------AUACGGCU-CUGa--C-GGCG- -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 23998 | 0.66 | 0.682174 |
Target: 5'- ---cUCGGCaagGCUGAGgaaaucaaGCUGCCGg -3' miRNA: 3'- cuaaAGCCGauaCGGCUC--------UGACGGCg -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 21114 | 0.66 | 0.682174 |
Target: 5'- ---cUCGGCguUGCCGAaacGACcGCCGa -3' miRNA: 3'- cuaaAGCCGauACGGCU---CUGaCGGCg -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 25713 | 0.67 | 0.647639 |
Target: 5'- cGAUUaCgGGCgcgGCCGuGGCaGCCGCc -3' miRNA: 3'- -CUAAaG-CCGauaCGGCuCUGaCGGCG- -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 31596 | 0.67 | 0.636079 |
Target: 5'- ----aCGGCUGgcaGCCGGGGCaacucgGCuCGCu -3' miRNA: 3'- cuaaaGCCGAUa--CGGCUCUGa-----CG-GCG- -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 5779 | 0.67 | 0.636079 |
Target: 5'- gGAgcgCGGCgggGCCGAc-CUGCUGCa -3' miRNA: 3'- -CUaaaGCCGauaCGGCUcuGACGGCG- -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 17808 | 0.67 | 0.624516 |
Target: 5'- ----aCGGCUGgggcUGCCGG---UGCCGCg -3' miRNA: 3'- cuaaaGCCGAU----ACGGCUcugACGGCG- -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 12543 | 0.67 | 0.61296 |
Target: 5'- ----gCGGCg--GCCGGcGCUGCgGCg -3' miRNA: 3'- cuaaaGCCGauaCGGCUcUGACGgCG- -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 21167 | 0.67 | 0.611806 |
Target: 5'- cGUUUCGGCaacGCCGAGcgccuucGCgccgGCUGCg -3' miRNA: 3'- cUAAAGCCGauaCGGCUC-------UGa---CGGCG- -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 6716 | 0.68 | 0.589915 |
Target: 5'- ---cUUGGCgcagcacGCCGAGACgcacggcgcGCCGCa -3' miRNA: 3'- cuaaAGCCGaua----CGGCUCUGa--------CGGCG- -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 26775 | 0.68 | 0.578445 |
Target: 5'- ----gCGGaUcgGCCGGGAUgggcgGCCGCg -3' miRNA: 3'- cuaaaGCCgAuaCGGCUCUGa----CGGCG- -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 29170 | 0.68 | 0.555663 |
Target: 5'- ---aUCGGCUAcGCCGGcGC-GCCGUu -3' miRNA: 3'- cuaaAGCCGAUaCGGCUcUGaCGGCG- -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 22166 | 0.69 | 0.544369 |
Target: 5'- -uUUUCGGCgggcGCCGuGAUUGUCGa -3' miRNA: 3'- cuAAAGCCGaua-CGGCuCUGACGGCg -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 17949 | 0.69 | 0.544369 |
Target: 5'- ---cUUGaGCgucGUGCCGAGGCggucgccgGCCGCc -3' miRNA: 3'- cuaaAGC-CGa--UACGGCUCUGa-------CGGCG- -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 4946 | 0.69 | 0.522014 |
Target: 5'- ---aUCGGCUuugucgagGUGCuuguacggguCGAGACgGCCGCc -3' miRNA: 3'- cuaaAGCCGA--------UACG----------GCUCUGaCGGCG- -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 24070 | 0.69 | 0.51097 |
Target: 5'- ---aUCGGCaagGUCGAG-CUGCCGg -3' miRNA: 3'- cuaaAGCCGauaCGGCUCuGACGGCg -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 33790 | 0.7 | 0.457352 |
Target: 5'- ---gUCGGaUGUGCUGAGAgUGgCGCu -3' miRNA: 3'- cuaaAGCCgAUACGGCUCUgACgGCG- -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 3651 | 0.71 | 0.426664 |
Target: 5'- ----cCGGCU-UGUCGAGACgGCCGa -3' miRNA: 3'- cuaaaGCCGAuACGGCUCUGaCGGCg -5' |
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27565 | 3' | -53.1 | NC_005882.1 | + | 17288 | 0.73 | 0.317154 |
Target: 5'- ---cUCGGCUAcUGCaUGAaGCUGCCGCc -3' miRNA: 3'- cuaaAGCCGAU-ACG-GCUcUGACGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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