miRNA display CGI


Results 1 - 10 of 10 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27567 3' -51.5 NC_005882.1 + 6869 0.66 0.78685
Target:  5'- aGAACgcgaAGAUCGAcGAGCUGGccGCgaagaaGACg -3'
miRNA:   3'- -CUUG----UCUAGCUuCUCGACCu-CGa-----CUG- -5'
27567 3' -51.5 NC_005882.1 + 32208 0.66 0.765355
Target:  5'- -cACGGcgCGAcuGAGCUGGcGCggGACg -3'
miRNA:   3'- cuUGUCuaGCUu-CUCGACCuCGa-CUG- -5'
27567 3' -51.5 NC_005882.1 + 10200 0.66 0.754364
Target:  5'- cGggUGGAUgCGAGccgcuccuggguGAGCUGGAGUUG-Cg -3'
miRNA:   3'- -CuuGUCUA-GCUU------------CUCGACCUCGACuG- -5'
27567 3' -51.5 NC_005882.1 + 11208 0.66 0.7477
Target:  5'- -cGCAGcgcgugcuccugaaaAUCGAAGAGCUGGGagaacauccGCUGcCa -3'
miRNA:   3'- cuUGUC---------------UAGCUUCUCGACCU---------CGACuG- -5'
27567 3' -51.5 NC_005882.1 + 16864 0.68 0.667163
Target:  5'- gGAACAGGUCGAcGAGauccaGGccggccgcgaccaaaAGCUGACg -3'
miRNA:   3'- -CUUGUCUAGCUuCUCga---CC---------------UCGACUG- -5'
27567 3' -51.5 NC_005882.1 + 10748 0.68 0.638888
Target:  5'- cGAGCGGcgaauGUCGAAGAGCUGGuuguGCc--- -3'
miRNA:   3'- -CUUGUC-----UAGCUUCUCGACCu---CGacug -5'
27567 3' -51.5 NC_005882.1 + 30859 0.71 0.498846
Target:  5'- uGAACGGAggugaugUCGGAGAGCUGGGucauGC-GGCc -3'
miRNA:   3'- -CUUGUCU-------AGCUUCUCGACCU----CGaCUG- -5'
27567 3' -51.5 NC_005882.1 + 2452 0.74 0.339513
Target:  5'- aAACgAGAUCGAAgGGGCUGGucgAGCUGAa -3'
miRNA:   3'- cUUG-UCUAGCUU-CUCGACC---UCGACUg -5'
27567 3' -51.5 NC_005882.1 + 24346 0.76 0.239146
Target:  5'- aAGCAGGUCaucgacggcgagGAAGuGCUGGAGCUGGa -3'
miRNA:   3'- cUUGUCUAG------------CUUCuCGACCUCGACUg -5'
27567 3' -51.5 NC_005882.1 + 7015 1.09 0.001131
Target:  5'- cGAACAGAUCGAAGAGCUGGAGCUGACc -3'
miRNA:   3'- -CUUGUCUAGCUUCUCGACCUCGACUG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.