miRNA: 3'- -CGUUUAUa-GG--CCGaCGGCGUuCCGCU- -5'
miRNA: 3'- -CGUUUaUAG-------------GCCGACGGCGU------UC-CGCU- -5'
miRNA: 3'- -CGUUUauaGG-------CCGA-CGGCGuUCCGCU- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2757 | 3' | -53.4 | NC_001491.2 | + | 123507 | 0.7 | 0.840173 |
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Target: 5'- cGCAGGUAcgCCgcGGCgGCUGCGuGGCGGg -3' miRNA: 3'- -CGUUUAUa-GG--CCGaCGGCGUuCCGCU- -5' |
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| 2757 | 3' | -53.4 | NC_001491.2 | + | 137182 | 0.7 | 0.839337 |
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Target: 5'- aGCAAAaAUCgugucugcugguaCGGCUGCCGCGaucucaGGaGCGAg -3' miRNA: 3'- -CGUUUaUAG-------------GCCGACGGCGU------UC-CGCU- -5' |
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| 2757 | 3' | -53.4 | NC_001491.2 | + | 147043 | 0.7 | 0.82221 |
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Target: 5'- cGCGGAg--CCggagaagGGCUcGCCGCcGGGCGAg -3' miRNA: 3'- -CGUUUauaGG-------CCGA-CGGCGuUCCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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