Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27572 | 3' | -58.3 | NC_005882.1 | + | 7760 | 0.66 | 0.466408 |
Target: 5'- aGCC-UCGACGCcGCCCgcgaGcgCCUCg- -3' miRNA: 3'- -CGGcAGCUGCGuCGGGa---CaaGGAGgu -5' |
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27572 | 3' | -58.3 | NC_005882.1 | + | 8887 | 0.66 | 0.456369 |
Target: 5'- uGCCGggUCGGCGCagauuucgccAGCCUUGagCC-CCAg -3' miRNA: 3'- -CGGC--AGCUGCG----------UCGGGACaaGGaGGU- -5' |
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27572 | 3' | -58.3 | NC_005882.1 | + | 31673 | 0.66 | 0.456369 |
Target: 5'- gGUCGUCGACGagcgAGCCgaGUUgCC-CCGg -3' miRNA: 3'- -CGGCAGCUGCg---UCGGgaCAA-GGaGGU- -5' |
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27572 | 3' | -58.3 | NC_005882.1 | + | 31392 | 0.66 | 0.435676 |
Target: 5'- gGCUGcCGGCGCAGCCCgcgaagacgacgcUGcgCUUCUc -3' miRNA: 3'- -CGGCaGCUGCGUCGGG-------------ACaaGGAGGu -5' |
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27572 | 3' | -58.3 | NC_005882.1 | + | 25162 | 0.66 | 0.426975 |
Target: 5'- gGCCGUCGugGcCGGCuCCUa--CCUCa- -3' miRNA: 3'- -CGGCAGCugC-GUCG-GGAcaaGGAGgu -5' |
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27572 | 3' | -58.3 | NC_005882.1 | + | 11198 | 0.67 | 0.408013 |
Target: 5'- uGCUGUCGGCGguGC-CUGUgCCUa-- -3' miRNA: 3'- -CGGCAGCUGCguCGgGACAaGGAggu -5' |
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27572 | 3' | -58.3 | NC_005882.1 | + | 22643 | 0.67 | 0.408013 |
Target: 5'- -aCGUCGACGCGGCgCaGUUCggCCc -3' miRNA: 3'- cgGCAGCUGCGUCGgGaCAAGgaGGu -5' |
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27572 | 3' | -58.3 | NC_005882.1 | + | 32404 | 0.67 | 0.398732 |
Target: 5'- uGgCGUCGACGCAuccGCaCUgccGUUCCUCgCAg -3' miRNA: 3'- -CgGCAGCUGCGU---CG-GGa--CAAGGAG-GU- -5' |
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27572 | 3' | -58.3 | NC_005882.1 | + | 28904 | 0.67 | 0.398732 |
Target: 5'- gGCCGaCGACGUAGCCgaUGaggagCUUCCGc -3' miRNA: 3'- -CGGCaGCUGCGUCGGg-ACaa---GGAGGU- -5' |
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27572 | 3' | -58.3 | NC_005882.1 | + | 22409 | 0.67 | 0.362991 |
Target: 5'- cGCCGUCGACcaAGCCCgcgcgCC-CCAg -3' miRNA: 3'- -CGGCAGCUGcgUCGGGacaa-GGaGGU- -5' |
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27572 | 3' | -58.3 | NC_005882.1 | + | 33398 | 0.67 | 0.360402 |
Target: 5'- uGCCGUCGugGCAauaCCCUGgaUgcuggaauacgacuCUCCAg -3' miRNA: 3'- -CGGCAGCugCGUc--GGGACaaG--------------GAGGU- -5' |
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27572 | 3' | -58.3 | NC_005882.1 | + | 14584 | 0.68 | 0.345973 |
Target: 5'- gGCCcaGUCGGCGCAGUCCcGgaugaaCUCCu -3' miRNA: 3'- -CGG--CAGCUGCGUCGGGaCaag---GAGGu -5' |
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27572 | 3' | -58.3 | NC_005882.1 | + | 28704 | 0.68 | 0.316803 |
Target: 5'- cGCCGUCGAgagcuggccCGCGGCCCgGUcgauugcgccgguaaUCgUCCc -3' miRNA: 3'- -CGGCAGCU---------GCGUCGGGaCA---------------AGgAGGu -5' |
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27572 | 3' | -58.3 | NC_005882.1 | + | 865 | 0.69 | 0.298408 |
Target: 5'- cGCaCGUCGACGCgcucgaauGGCUCaGUUCCgcCCAg -3' miRNA: 3'- -CG-GCAGCUGCG--------UCGGGaCAAGGa-GGU- -5' |
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27572 | 3' | -58.3 | NC_005882.1 | + | 28380 | 0.77 | 0.080092 |
Target: 5'- gGCCGUCGACGCGGUCaaGgUCgUCCAg -3' miRNA: 3'- -CGGCAGCUGCGUCGGgaCaAGgAGGU- -5' |
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27572 | 3' | -58.3 | NC_005882.1 | + | 8704 | 1.1 | 0.000235 |
Target: 5'- cGCCGUCGACGCAGCCCUGUUCCUCCAg -3' miRNA: 3'- -CGGCAGCUGCGUCGGGACAAGGAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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