Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27574 | 3' | -54.9 | NC_005882.1 | + | 3534 | 0.66 | 0.63531 |
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Target: 5'- -uUCCgACCGCGAGCGGguACa---- -3' miRNA: 3'- gcAGGgUGGUGCUUGCCguUGgauca -5' |
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| 27574 | 3' | -54.9 | NC_005882.1 | + | 14406 | 0.66 | 0.612669 |
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Target: 5'- aGUCgCGCCGCGu-UGGCuuguCCUGGg -3' miRNA: 3'- gCAGgGUGGUGCuuGCCGuu--GGAUCa -5' |
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| 27574 | 3' | -54.9 | NC_005882.1 | + | 26836 | 0.67 | 0.567678 |
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Target: 5'- aG-CCgGCCGCGAGCGucGCGACCUu-- -3' miRNA: 3'- gCaGGgUGGUGCUUGC--CGUUGGAuca -5' |
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| 27574 | 3' | -54.9 | NC_005882.1 | + | 27146 | 0.68 | 0.512735 |
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Target: 5'- -aUCCCGCCcUGGGCGaGCAGCCg--- -3' miRNA: 3'- gcAGGGUGGuGCUUGC-CGUUGGauca -5' |
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| 27574 | 3' | -54.9 | NC_005882.1 | + | 20730 | 0.69 | 0.449921 |
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Target: 5'- aCGUUCCGCUGCaGcGCGGCGGCCa--- -3' miRNA: 3'- -GCAGGGUGGUG-CuUGCCGUUGGauca -5' |
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| 27574 | 3' | -54.9 | NC_005882.1 | + | 3332 | 0.73 | 0.268838 |
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Target: 5'- -aUCUCGCCGCGcGACGGCGGCCg--- -3' miRNA: 3'- gcAGGGUGGUGC-UUGCCGUUGGauca -5' |
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| 27574 | 3' | -54.9 | NC_005882.1 | + | 9195 | 1.07 | 0.00093 |
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Target: 5'- cCGUCCCACCACGAACGGCAACCUAGUc -3' miRNA: 3'- -GCAGGGUGGUGCUUGCCGUUGGAUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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