Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27574 | 5' | -55.4 | NC_005882.1 | + | 26118 | 0.66 | 0.624268 |
Target: 5'- gCCGCGGUcauuuCCGGCAGagCCUUGGCc- -3' miRNA: 3'- -GGUGCCAc----GGCUGUCggGGAAUUGuc -5' |
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27574 | 5' | -55.4 | NC_005882.1 | + | 30118 | 0.66 | 0.62314 |
Target: 5'- uUCGCGGUgacGCCGuagaccuGCAGCCCUuucgUUGACu- -3' miRNA: 3'- -GGUGCCA---CGGC-------UGUCGGGG----AAUUGuc -5' |
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27574 | 5' | -55.4 | NC_005882.1 | + | 31449 | 0.67 | 0.557106 |
Target: 5'- uUCGCGGgcugcGCCGGCAGCCgCgucACGc -3' miRNA: 3'- -GGUGCCa----CGGCUGUCGGgGaauUGUc -5' |
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27574 | 5' | -55.4 | NC_005882.1 | + | 30320 | 0.67 | 0.546081 |
Target: 5'- gUCGCGGUGCUccugGACAGCCggugCgagGACGGu -3' miRNA: 3'- -GGUGCCACGG----CUGUCGGg---Gaa-UUGUC- -5' |
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27574 | 5' | -55.4 | NC_005882.1 | + | 30076 | 0.67 | 0.535128 |
Target: 5'- uUCGCGGUGCCGccGCcuuuGCCggCCUgGACGGc -3' miRNA: 3'- -GGUGCCACGGC--UGu---CGG--GGAaUUGUC- -5' |
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27574 | 5' | -55.4 | NC_005882.1 | + | 28258 | 0.67 | 0.513462 |
Target: 5'- cCCAaGGUGCCGccgGCGGCCgCgcaGGCGGu -3' miRNA: 3'- -GGUgCCACGGC---UGUCGGgGaa-UUGUC- -5' |
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27574 | 5' | -55.4 | NC_005882.1 | + | 11984 | 0.68 | 0.502764 |
Target: 5'- gCCACGG-GCgCGGCGGCgCCga-GCAa -3' miRNA: 3'- -GGUGCCaCG-GCUGUCGgGGaauUGUc -5' |
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27574 | 5' | -55.4 | NC_005882.1 | + | 22638 | 0.68 | 0.492165 |
Target: 5'- -uGCGGUGCCGAuCAagccgaucaccGCCgacuucacgaCCUUGACGGg -3' miRNA: 3'- ggUGCCACGGCU-GU-----------CGG----------GGAAUUGUC- -5' |
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27574 | 5' | -55.4 | NC_005882.1 | + | 12333 | 0.68 | 0.471284 |
Target: 5'- -gACGGcUGCCGGCGGCgCCgaucGGCAa -3' miRNA: 3'- ggUGCC-ACGGCUGUCGgGGaa--UUGUc -5' |
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27574 | 5' | -55.4 | NC_005882.1 | + | 28318 | 0.68 | 0.471284 |
Target: 5'- gCGCGGccGCCGGCGGCaCCUUGggcuguACGGc -3' miRNA: 3'- gGUGCCa-CGGCUGUCGgGGAAU------UGUC- -5' |
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27574 | 5' | -55.4 | NC_005882.1 | + | 13002 | 0.69 | 0.440834 |
Target: 5'- gCGCGG-GCCGgcgcaACAGCUCCggcGGCAGc -3' miRNA: 3'- gGUGCCaCGGC-----UGUCGGGGaa-UUGUC- -5' |
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27574 | 5' | -55.4 | NC_005882.1 | + | 21667 | 0.69 | 0.430935 |
Target: 5'- aCACGGUGuCCGggagcGCGGCgCCUUccggucGACGGg -3' miRNA: 3'- gGUGCCAC-GGC-----UGUCGgGGAA------UUGUC- -5' |
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27574 | 5' | -55.4 | NC_005882.1 | + | 17150 | 0.69 | 0.430935 |
Target: 5'- gCGCGaGUGCCGACgAGCUgCUcGGCAu -3' miRNA: 3'- gGUGC-CACGGCUG-UCGGgGAaUUGUc -5' |
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27574 | 5' | -55.4 | NC_005882.1 | + | 31388 | 0.69 | 0.421167 |
Target: 5'- aCGCGGcUGCCGgcGCAGCCCgCgaaGACGa -3' miRNA: 3'- gGUGCC-ACGGC--UGUCGGG-Gaa-UUGUc -5' |
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27574 | 5' | -55.4 | NC_005882.1 | + | 27653 | 0.7 | 0.36548 |
Target: 5'- gCCGCGGccugcgucgccaUGCCGGCGGCCgCgcgcGCGGc -3' miRNA: 3'- -GGUGCC------------ACGGCUGUCGGgGaau-UGUC- -5' |
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27574 | 5' | -55.4 | NC_005882.1 | + | 17867 | 0.71 | 0.315087 |
Target: 5'- aCGCGGcaCCGGCAGCCCCagccguucGGCGGg -3' miRNA: 3'- gGUGCCacGGCUGUCGGGGaa------UUGUC- -5' |
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27574 | 5' | -55.4 | NC_005882.1 | + | 9160 | 1.11 | 0.000432 |
Target: 5'- uCCACGGUGCCGACAGCCCCUUAACAGg -3' miRNA: 3'- -GGUGCCACGGCUGUCGGGGAAUUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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