miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27577 3' -58.6 NC_005882.1 + 18214 0.66 0.439719
Target:  5'- uGGCCGGCUUGuUCGagcugcagcaagcgcGUGCgcaGCGcCGa -3'
miRNA:   3'- gCCGGCCGAGC-AGU---------------CACGaa-CGCaGC- -5'
27577 3' -58.6 NC_005882.1 + 10830 0.66 0.416699
Target:  5'- gCGGUCGcGCUCGUCGcUGCgggGC-UCGu -3'
miRNA:   3'- -GCCGGC-CGAGCAGUcACGaa-CGcAGC- -5'
27577 3' -58.6 NC_005882.1 + 9730 0.66 0.407326
Target:  5'- gGGCCGGCUCaaUCGGgaGCgUUGCugGUCGc -3'
miRNA:   3'- gCCGGCCGAGc-AGUCa-CG-AACG--CAGC- -5'
27577 3' -58.6 NC_005882.1 + 6104 0.66 0.407326
Target:  5'- aCGGCCGcaGCUCGcCGGUGgacuCGUCGa -3'
miRNA:   3'- -GCCGGC--CGAGCaGUCACgaacGCAGC- -5'
27577 3' -58.6 NC_005882.1 + 29421 0.66 0.407326
Target:  5'- gCGGCCGGUgagCGUCAGgugGUUccGCuUCGc -3'
miRNA:   3'- -GCCGGCCGa--GCAGUCa--CGAa-CGcAGC- -5'
27577 3' -58.6 NC_005882.1 + 25103 0.67 0.383587
Target:  5'- gCGGCCGGCUCgggcGccgggccgccugacaUCAGgauUGCUUugucGCGUCGa -3'
miRNA:   3'- -GCCGGCCGAG----C---------------AGUC---ACGAA----CGCAGC- -5'
27577 3' -58.6 NC_005882.1 + 32017 0.67 0.362505
Target:  5'- gCGGCCGccgacGCUCGUCGGUcagGUUgucGCGUaCGa -3'
miRNA:   3'- -GCCGGC-----CGAGCAGUCA---CGAa--CGCA-GC- -5'
27577 3' -58.6 NC_005882.1 + 31581 0.67 0.353961
Target:  5'- uGGCCGGCgacgagCGUCAc-GUcgGUGUCGg -3'
miRNA:   3'- gCCGGCCGa-----GCAGUcaCGaaCGCAGC- -5'
27577 3' -58.6 NC_005882.1 + 33332 0.68 0.321225
Target:  5'- -cGCCaGCgcCGUCGaguuaucccccuGUGCUUGCGUCGg -3'
miRNA:   3'- gcCGGcCGa-GCAGU------------CACGAACGCAGC- -5'
27577 3' -58.6 NC_005882.1 + 648 0.71 0.196721
Target:  5'- uCGGCCaGCUCGaUCAGUGCcUGgG-CGa -3'
miRNA:   3'- -GCCGGcCGAGC-AGUCACGaACgCaGC- -5'
27577 3' -58.6 NC_005882.1 + 10459 1.08 0.000335
Target:  5'- cCGGCCGGCUCGUCAGUGCUUGCGUCGu -3'
miRNA:   3'- -GCCGGCCGAGCAGUCACGAACGCAGC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.