Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27580 | 3' | -56.9 | NC_005882.1 | + | 28233 | 0.66 | 0.515017 |
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Target: 5'- cCCGUUcaagcCGCcgGCcguacagcccaagGUGCCGCCGGCGGCc -3' miRNA: 3'- -GGCAA-----GCGa-CG-------------CGUGGCGGUCGUUGu -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 28332 | 0.76 | 0.116388 |
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Target: 5'- gCUGgagacCGcCUGCGCgGCCGCCGGCGGCAc -3' miRNA: 3'- -GGCaa---GC-GACGCG-UGGCGGUCGUUGU- -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 30200 | 0.66 | 0.494847 |
|
Target: 5'- cCCGgcuUCGCgcGCGUACgGCUgaccgaccucGGCAACAu -3' miRNA: 3'- -GGCa--AGCGa-CGCGUGgCGG----------UCGUUGU- -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 30524 | 0.66 | 0.526846 |
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Target: 5'- ----aUGCUGCGCGCgGCCgacaaGGUGACGg -3' miRNA: 3'- ggcaaGCGACGCGUGgCGG-----UCGUUGU- -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 30628 | 0.66 | 0.516088 |
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Target: 5'- aUCGggCgGCgaugGCGCugCGCUgacgGGCAACGu -3' miRNA: 3'- -GGCaaG-CGa---CGCGugGCGG----UCGUUGU- -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 31455 | 0.73 | 0.177601 |
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Target: 5'- gUCGUcuUCGC-GgGCugCGCCGGCAGCc -3' miRNA: 3'- -GGCA--AGCGaCgCGugGCGGUCGUUGu -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 32132 | 0.66 | 0.523609 |
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Target: 5'- gCCGUacgugccugcgcgaUCGUUGUGuCGgCGCCGGCAu-- -3' miRNA: 3'- -GGCA--------------AGCGACGC-GUgGCGGUCGUugu -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 32202 | 0.73 | 0.187654 |
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Target: 5'- gCCGggacUCGCacUGCGCgugcugACCGCCAGCGAa- -3' miRNA: 3'- -GGCa---AGCG--ACGCG------UGGCGGUCGUUgu -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 32747 | 0.71 | 0.258953 |
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Target: 5'- aCGUUgGCUGCGUuCgCGCCGGCucgauGCGu -3' miRNA: 3'- gGCAAgCGACGCGuG-GCGGUCGu----UGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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