Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27580 | 3' | -56.9 | NC_005882.1 | + | 6402 | 0.77 | 0.100786 |
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Target: 5'- gCCGgcCGC-GCGCGCUGCCugGGCGACAg -3' miRNA: 3'- -GGCaaGCGaCGCGUGGCGG--UCGUUGU- -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 5926 | 0.66 | 0.494847 |
|
Target: 5'- gCCGUUCGCacGgGCAUCGgcuaCCGGCuuCAg -3' miRNA: 3'- -GGCAAGCGa-CgCGUGGC----GGUCGuuGU- -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 5070 | 0.72 | 0.225049 |
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Target: 5'- gCGcaUGCUGCGCuggcguaucggcguGCCGCCGGUAGCu -3' miRNA: 3'- gGCaaGCGACGCG--------------UGGCGGUCGUUGu -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 3662 | 0.67 | 0.463762 |
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Target: 5'- gCGUcgacgCGCUGCGCACuCGgcucacgaugaCCAGCAcGCGa -3' miRNA: 3'- gGCAa----GCGACGCGUG-GC-----------GGUCGU-UGU- -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 3376 | 0.67 | 0.433729 |
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Target: 5'- uCCGcgUCGaaaacccGCGUGCCGUCAgGCAGCAg -3' miRNA: 3'- -GGCa-AGCga-----CGCGUGGCGGU-CGUUGU- -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 3375 | 0.67 | 0.453628 |
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Target: 5'- gCGggCGCUGCGCggccugggcuACCGCguGCuGGCc -3' miRNA: 3'- gGCaaGCGACGCG----------UGGCGguCG-UUGu -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 3272 | 0.67 | 0.443615 |
|
Target: 5'- uCCGacgUGCUGCGCGagcgUGCgGGCGGCGu -3' miRNA: 3'- -GGCaa-GCGACGCGUg---GCGgUCGUUGU- -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 2712 | 0.71 | 0.272793 |
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Target: 5'- uCCGgaCGCUGCGCAUUgaagGCCAGUuucucAACGa -3' miRNA: 3'- -GGCaaGCGACGCGUGG----CGGUCG-----UUGU- -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 1366 | 0.73 | 0.198205 |
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Target: 5'- gCUGUUUGCUGCGCGCUuCCucGCGGCc -3' miRNA: 3'- -GGCAAGCGACGCGUGGcGGu-CGUUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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