Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27580 | 3' | -56.9 | NC_005882.1 | + | 16540 | 0.71 | 0.258953 |
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Target: 5'- aUCGgaugCGCUGCGCggaugcuuGCCGaCCAGCAucgGCGa -3' miRNA: 3'- -GGCaa--GCGACGCG--------UGGC-GGUCGU---UGU- -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 12605 | 0.72 | 0.226863 |
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Target: 5'- gCCGaUCGCcgcaGCGCcgGCCGCCGccGCGACAc -3' miRNA: 3'- -GGCaAGCGa---CGCG--UGGCGGU--CGUUGU- -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 1366 | 0.73 | 0.198205 |
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Target: 5'- gCUGUUUGCUGCGCGCUuCCucGCGGCc -3' miRNA: 3'- -GGCAAGCGACGCGUGGcGGu-CGUUGu -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 20800 | 0.73 | 0.198205 |
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Target: 5'- gCGUaCGCUGUugGCCGCCGGCuGCAg -3' miRNA: 3'- gGCAaGCGACGcgUGGCGGUCGuUGU- -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 31455 | 0.73 | 0.177601 |
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Target: 5'- gUCGUcuUCGC-GgGCugCGCCGGCAGCc -3' miRNA: 3'- -GGCA--AGCGaCgCGugGCGGUCGUUGu -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 12408 | 0.75 | 0.146095 |
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Target: 5'- gCCGaaccagCGUUGCcgaucgGCGCCGCCGGCAGCc -3' miRNA: 3'- -GGCaa----GCGACG------CGUGGCGGUCGUUGu -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 28332 | 0.76 | 0.116388 |
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Target: 5'- gCUGgagacCGcCUGCGCgGCCGCCGGCGGCAc -3' miRNA: 3'- -GGCaa---GC-GACGCG-UGGCGGUCGUUGU- -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 11045 | 1.1 | 0.000354 |
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Target: 5'- gCCGUUCGCUGCGCACCGCCAGCAACAc -3' miRNA: 3'- -GGCAAGCGACGCGUGGCGGUCGUUGU- -5' |
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| 27580 | 3' | -56.9 | NC_005882.1 | + | 32132 | 0.66 | 0.523609 |
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Target: 5'- gCCGUacgugccugcgcgaUCGUUGUGuCGgCGCCGGCAu-- -3' miRNA: 3'- -GGCA--------------AGCGACGC-GUgGCGGUCGUugu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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