Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27582 | 5' | -49.1 | NC_005882.1 | + | 30850 | 0.66 | 0.89666 |
Target: 5'- gGCGUUGUugAGgGCguGGAugacaucgaUCAGGc- -3' miRNA: 3'- -CGCAACAugUCgCGguCUU---------AGUCUau -5' |
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27582 | 5' | -49.1 | NC_005882.1 | + | 27727 | 0.66 | 0.895125 |
Target: 5'- gGCGgcucaccggACGGCGUCaAGGAUCAGGUc -3' miRNA: 3'- -CGCaaca-----UGUCGCGG-UCUUAGUCUAu -5' |
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27582 | 5' | -49.1 | NC_005882.1 | + | 32113 | 0.66 | 0.888863 |
Target: 5'- uCGUUGUGuCGGCGCCGGcAUCc---- -3' miRNA: 3'- cGCAACAU-GUCGCGGUCuUAGucuau -5' |
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27582 | 5' | -49.1 | NC_005882.1 | + | 18187 | 0.67 | 0.872348 |
Target: 5'- cGCGUgc-GCAGCGCC-GAGUCGucGAUc -3' miRNA: 3'- -CGCAacaUGUCGCGGuCUUAGU--CUAu -5' |
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27582 | 5' | -49.1 | NC_005882.1 | + | 13556 | 1.1 | 0.001997 |
Target: 5'- cGCGUUGUACAGCGCCAGAAUCAGAUAc -3' miRNA: 3'- -CGCAACAUGUCGCGGUCUUAGUCUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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