Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 27583 | 3' | -59.4 | NC_005882.1 | + | 11991 | 0.72 | 0.158265 |
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Target: 5'- uUGCGCggccacgGGCgcGGCggcGcCGAGCAACGCg -3' miRNA: 3'- gGCGCGa------CCGa-CCGa--C-GCUCGUUGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 23228 | 0.73 | 0.123223 |
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Target: 5'- -gGCGCUGGCcgGGC-GCGGGcCGGCGg -3' miRNA: 3'- ggCGCGACCGa-CCGaCGCUC-GUUGCg -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 28039 | 0.73 | 0.134021 |
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Target: 5'- gCCGaGCUGGCgaaGCUGCGcGCGGCGg -3' miRNA: 3'- -GGCgCGACCGac-CGACGCuCGUUGCg -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 11968 | 0.74 | 0.11648 |
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Target: 5'- -gGCGCUGGCUaccgcgccGaGCUGaCGcGCAACGCa -3' miRNA: 3'- ggCGCGACCGA--------C-CGAC-GCuCGUUGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 22450 | 0.74 | 0.100239 |
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Target: 5'- aCgGCGCUgcGGCcGGCUucagguagcccaucGCGAGCAGCGUc -3' miRNA: 3'- -GgCGCGA--CCGaCCGA--------------CGCUCGUUGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 25581 | 0.75 | 0.098261 |
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Target: 5'- gCGCGC-GGCgccGcGCUGCguGAGCAACGCc -3' miRNA: 3'- gGCGCGaCCGa--C-CGACG--CUCGUUGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 9500 | 0.77 | 0.069605 |
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Target: 5'- gCGCGCUGGC-GGCcGCaAGCAcgACGCg -3' miRNA: 3'- gGCGCGACCGaCCGaCGcUCGU--UGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 2100 | 0.84 | 0.01965 |
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Target: 5'- uCCGCGCUGGCUGGaggcaaUGCGAGCcaucGGCGa -3' miRNA: 3'- -GGCGCGACCGACCg-----ACGCUCG----UUGCg -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 14512 | 1.12 | 0.000124 |
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Target: 5'- gCCGCGCUGGCUGGCUGCGAGCAACGCu -3' miRNA: 3'- -GGCGCGACCGACCGACGCUCGUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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