Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27583 | 3' | -59.4 | NC_005882.1 | + | 20796 | 0.67 | 0.345561 |
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Target: 5'- aCGCuGUUGGCcgccGGCUGC-AGCGgaACGUa -3' miRNA: 3'- gGCG-CGACCGa---CCGACGcUCGU--UGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 13251 | 0.66 | 0.380017 |
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Target: 5'- aUCGCGCUcGGaCUGGUggcgcucgaucUGCacGAGCAACa- -3' miRNA: 3'- -GGCGCGA-CC-GACCG-----------ACG--CUCGUUGcg -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 3585 | 0.66 | 0.380017 |
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Target: 5'- gCGUGCUGGUcaucgUGaGCcgaGUGcGCAGCGCg -3' miRNA: 3'- gGCGCGACCG-----AC-CGa--CGCuCGUUGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 27873 | 0.66 | 0.388081 |
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Target: 5'- aCGCGC-GGCgcucGcGCUucGCGAGCAggucgucgagcauGCGCu -3' miRNA: 3'- gGCGCGaCCGa---C-CGA--CGCUCGU-------------UGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 10980 | 0.66 | 0.388983 |
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Target: 5'- gCGUGUU-GCUGGCgGUGcGCAGCGa -3' miRNA: 3'- gGCGCGAcCGACCGaCGCuCGUUGCg -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 25444 | 0.66 | 0.388983 |
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Target: 5'- cCCGC-CUGGgggacgcgaUGGC-GCGGGCcguuGCGCa -3' miRNA: 3'- -GGCGcGACCg--------ACCGaCGCUCGu---UGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 26148 | 0.66 | 0.388983 |
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Target: 5'- gCCGCGaaGGCgccGGCaGUG-GCGACGa -3' miRNA: 3'- -GGCGCgaCCGa--CCGaCGCuCGUUGCg -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 16718 | 0.66 | 0.398087 |
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Target: 5'- uCCGCGCaGGCcgGGCUugagGUGAcggacgacauucGCGACGg -3' miRNA: 3'- -GGCGCGaCCGa-CCGA----CGCU------------CGUUGCg -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 3656 | 0.66 | 0.398087 |
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Target: 5'- aCGCGCUGcGCacucGGCUcaCGAugaccagcacGCGACGCg -3' miRNA: 3'- gGCGCGAC-CGa---CCGAc-GCU----------CGUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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