Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27583 | 3' | -59.4 | NC_005882.1 | + | 9500 | 0.77 | 0.069605 |
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Target: 5'- gCGCGCUGGC-GGCcGCaAGCAcgACGCg -3' miRNA: 3'- gGCGCGACCGaCCGaCGcUCGU--UGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 8776 | 0.69 | 0.253388 |
|
Target: 5'- cCCGUGCcuguaucggaucgGGCaguUGGC-GaCGAGCAGCGCc -3' miRNA: 3'- -GGCGCGa------------CCG---ACCGaC-GCUCGUUGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 8432 | 0.68 | 0.282848 |
|
Target: 5'- aCGCGCUugaacucuguaaaGaGCaUGcGC-GCGAGCGGCGCa -3' miRNA: 3'- gGCGCGA-------------C-CG-AC-CGaCGCUCGUUGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 6690 | 0.67 | 0.34059 |
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Target: 5'- gCGCGCcgcacgacgacuucaUGGCUGGCguccuCGGGCAAauccaguuguCGCu -3' miRNA: 3'- gGCGCG---------------ACCGACCGac---GCUCGUU----------GCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 3656 | 0.66 | 0.398087 |
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Target: 5'- aCGCGCUGcGCacucGGCUcaCGAugaccagcacGCGACGCg -3' miRNA: 3'- gGCGCGAC-CGa---CCGAc-GCU----------CGUUGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 3585 | 0.66 | 0.380017 |
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Target: 5'- gCGUGCUGGUcaucgUGaGCcgaGUGcGCAGCGCg -3' miRNA: 3'- gGCGCGACCG-----AC-CGa--CGCuCGUUGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 3268 | 0.71 | 0.16719 |
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Target: 5'- aCGUGCUGcGC-GaGCgUGCGGGCGGCGUc -3' miRNA: 3'- gGCGCGAC-CGaC-CG-ACGCUCGUUGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 2100 | 0.84 | 0.01965 |
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Target: 5'- uCCGCGCUGGCUGGaggcaaUGCGAGCcaucGGCGa -3' miRNA: 3'- -GGCGCGACCGACCg-----ACGCUCG----UUGCg -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 1962 | 0.7 | 0.203692 |
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Target: 5'- aCCGCGacaucgagcacuuucGCUGGCcGCGAGCGucuCGCg -3' miRNA: 3'- -GGCGCgac------------CGACCGaCGCUCGUu--GCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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