Results 41 - 49 of 49 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27583 | 3' | -59.4 | NC_005882.1 | + | 3268 | 0.71 | 0.16719 |
Target: 5'- aCGUGCUGcGC-GaGCgUGCGGGCGGCGUc -3' miRNA: 3'- gGCGCGAC-CGaC-CG-ACGCUCGUUGCG- -5' |
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27583 | 3' | -59.4 | NC_005882.1 | + | 1962 | 0.7 | 0.203692 |
Target: 5'- aCCGCGacaucgagcacuuucGCUGGCcGCGAGCGucuCGCg -3' miRNA: 3'- -GGCGCgac------------CGACCGaCGCUCGUu--GCG- -5' |
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27583 | 3' | -59.4 | NC_005882.1 | + | 32204 | 0.7 | 0.207534 |
Target: 5'- gCGCGaCUGaGCUGGC-GCGGGaCGAuauCGCg -3' miRNA: 3'- gGCGC-GAC-CGACCGaCGCUC-GUU---GCG- -5' |
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27583 | 3' | -59.4 | NC_005882.1 | + | 32284 | 0.69 | 0.230704 |
Target: 5'- aCCGCGCccauucGCUGGCgGUcAGCAcGCGCa -3' miRNA: 3'- -GGCGCGac----CGACCGaCGcUCGU-UGCG- -5' |
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27583 | 3' | -59.4 | NC_005882.1 | + | 14972 | 0.69 | 0.236828 |
Target: 5'- aUGCGCUGGUagagcccguccUGGaCUGCGucGGCgAACGUg -3' miRNA: 3'- gGCGCGACCG-----------ACC-GACGC--UCG-UUGCG- -5' |
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27583 | 3' | -59.4 | NC_005882.1 | + | 10679 | 0.69 | 0.249484 |
Target: 5'- cCCGUGUcGGCagcGGCggagGCGgcGGCAGCGUg -3' miRNA: 3'- -GGCGCGaCCGa--CCGa---CGC--UCGUUGCG- -5' |
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27583 | 3' | -59.4 | NC_005882.1 | + | 8776 | 0.69 | 0.253388 |
Target: 5'- cCCGUGCcuguaucggaucgGGCaguUGGC-GaCGAGCAGCGCc -3' miRNA: 3'- -GGCGCGa------------CCG---ACCGaC-GCUCGUUGCG- -5' |
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27583 | 3' | -59.4 | NC_005882.1 | + | 14737 | 0.69 | 0.262693 |
Target: 5'- gCCGCGCaGGUUGGaCgggcGCGAcgacagugccGUGACGCg -3' miRNA: 3'- -GGCGCGaCCGACC-Ga---CGCU----------CGUUGCG- -5' |
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27583 | 3' | -59.4 | NC_005882.1 | + | 14512 | 1.12 | 0.000124 |
Target: 5'- gCCGCGCUGGCUGGCUGCGAGCAACGCu -3' miRNA: 3'- -GGCGCGACCGACCGACGCUCGUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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