Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27583 | 3' | -59.4 | NC_005882.1 | + | 26148 | 0.66 | 0.388983 |
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Target: 5'- gCCGCGaaGGCgccGGCaGUG-GCGACGa -3' miRNA: 3'- -GGCGCgaCCGa--CCGaCGCuCGUUGCg -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 3585 | 0.66 | 0.380017 |
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Target: 5'- gCGUGCUGGUcaucgUGaGCcgaGUGcGCAGCGCg -3' miRNA: 3'- gGCGCGACCG-----AC-CGa--CGCuCGUUGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 10980 | 0.66 | 0.388983 |
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Target: 5'- gCGUGUU-GCUGGCgGUGcGCAGCGa -3' miRNA: 3'- gGCGCGAcCGACCGaCGCuCGUUGCg -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 14112 | 0.67 | 0.329192 |
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Target: 5'- aUCGCGCU-GCUguccaaGGCcGCGAagaacaucGCGACGCu -3' miRNA: 3'- -GGCGCGAcCGA------CCGaCGCU--------CGUUGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 14412 | 0.67 | 0.329192 |
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Target: 5'- gCCGCGUUGGCuuguccUGGg-GCGAaGCAGC-Cg -3' miRNA: 3'- -GGCGCGACCG------ACCgaCGCU-CGUUGcG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 15056 | 0.67 | 0.321225 |
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Target: 5'- gCgGCGCaUGGCUGucgcgcgcGCUGauCGAGUcGCGCa -3' miRNA: 3'- -GgCGCG-ACCGAC--------CGAC--GCUCGuUGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 27165 | 0.68 | 0.305727 |
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Target: 5'- gCGCGC-GGUgcucUGGCU-CGGGC-GCGCg -3' miRNA: 3'- gGCGCGaCCG----ACCGAcGCUCGuUGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 26568 | 0.68 | 0.305727 |
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Target: 5'- gCCGCGCgGaGCUGGCgaccgucGCGuggGGCcgcuuucaGACGCa -3' miRNA: 3'- -GGCGCGaC-CGACCGa------CGC---UCG--------UUGCG- -5' |
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| 27583 | 3' | -59.4 | NC_005882.1 | + | 9500 | 0.77 | 0.069605 |
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Target: 5'- gCGCGCUGGC-GGCcGCaAGCAcgACGCg -3' miRNA: 3'- gGCGCGACCGaCCGaCGcUCGU--UGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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