miRNA: 3'- -GUG-GCCcaGGCC-----------UAAGCCGUCCAUgU- -5'
miRNA: 3'- gUGGCCCaG-GCCUaaGCCGUCCaUGU- -5'
miRNA: 3'- gUGGCCCAGGCCUaaGCCgUC-CAUGu -5'
miRNA: 3'- gUGGCC-CAGGCcuaaGC-CGUCCAUGU- -5'
miRNA: 3'- -GUGGCCCAGGCCUAAGCCGUCCAUGU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27586 | 5' | -57.5 | NC_005882.1 | + | 17869 | 0.66 | 0.496409 |
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Target: 5'- cCACgCGGcaCCGGcagccccagccGUUCGGCGGGUAgAa -3' miRNA: 3'- -GUG-GCCcaGGCC-----------UAAGCCGUCCAUgU- -5' |
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| 27586 | 5' | -57.5 | NC_005882.1 | + | 23234 | 0.66 | 0.475717 |
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Target: 5'- gGCCGGG-CgCGGGc-CGGCGGGcACGa -3' miRNA: 3'- gUGGCCCaG-GCCUaaGCCGUCCaUGU- -5' |
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| 27586 | 5' | -57.5 | NC_005882.1 | + | 12474 | 0.69 | 0.304734 |
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Target: 5'- gGCCGGGUUCGGGc-CGGgAGcGUGCu -3' miRNA: 3'- gUGGCCCAGGCCUaaGCCgUC-CAUGu -5' |
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| 27586 | 5' | -57.5 | NC_005882.1 | + | 20938 | 0.7 | 0.282463 |
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Target: 5'- aGCCGGcGUCCGcgc-CGaGCAGGUACGg -3' miRNA: 3'- gUGGCC-CAGGCcuaaGC-CGUCCAUGU- -5' |
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| 27586 | 5' | -57.5 | NC_005882.1 | + | 15983 | 1.08 | 0.000468 |
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Target: 5'- gCACCGGGUCCGGAUUCGGCAGGUACAu -3' miRNA: 3'- -GUGGCCCAGGCCUAAGCCGUCCAUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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